Journal article
ROCK Inhibition Enhances the Recovery and Growth of Cryopreserved Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells
Molecular reproduction and development, Vol.76(8), pp.722-732
2009
DOI: 10.1002/mrd.21021
PMCID: PMC3257892
PMID: 19235204
Abstract
Poor recovery of cryopreserved human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells is a significant impediment to progress with pluripotent stem cells. In this study, we demonstrate that Y-27632, a specific inhibitor of Rho kinase (ROCK) activity, significantly enhances recovery of hES cells from cryopreserved stocks when cultured with or without a growth inactivated feeder layer. Furthermore, treatment with the ROCK inhibitor for several days increased the number of colonies and colony size of hES cells compared to shorter exposures. Remarkably, hES cells that had formed relatively few colonies 5 days after thawing exhibited rapid growth upon addition of Y-27632. Additionally, we determined that Y-27632 significantly improves the recovery of cryopreserved human iPS cells and their growth upon subculture. Thus, Y-27632 provides a means to "kick-start" slow-growing human pluripotent stem cells, especially after being thawed from frozen stocks. Together, these results argue that Y-27632 is a useful tool in overcoming obstacles to studies involving the cultivation of both hES cells and human iPS cells.
Details
- Title: Subtitle
- ROCK Inhibition Enhances the Recovery and Growth of Cryopreserved Human Embryonic Stem Cells and Human Induced Pluripotent Stem Cells
- Creators
- David A CLAASSEN - Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, United StatesMichelle M DESLER - Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, United StatesAngie RIZZINO - Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, United States
- Resource Type
- Journal article
- Publication Details
- Molecular reproduction and development, Vol.76(8), pp.722-732
- DOI
- 10.1002/mrd.21021
- PMID
- 19235204
- PMCID
- PMC3257892
- NLM abbreviation
- Mol Reprod Dev
- ISSN
- 1040-452X
- eISSN
- 1098-2795
- Publisher
- Wiley-Liss
- Language
- English
- Date published
- 2009
- Academic Unit
- Stead Family Department of Pediatrics; Hematology/Oncology
- Record Identifier
- 9984093213802771
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