Journal article
RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis
Nucleic acids research, Vol.49(4), pp.2005-2026
02/26/2021
DOI: 10.1093/nar/gkaa1293
PMCID: PMC7913698
PMID: 33476370
Abstract
Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome encodes RPA-1, RPA-2 and an RPA-2 paralog RPA-4. In our analysis, we determined that RPA-2 is critical for germline replication and normal repair of meiotic DSBs. Interestingly, RPA-1 but not RPA-2 is essential for somatic replication, in contrast to other organisms that require both subunits. Six different hetero- and homodimeric complexes containing permutations of RPA-1, RPA-2 and RPA-4 can be detected in whole animal extracts. Our in vivo studies indicate that RPA-1/4 dimer is less abundant in the nucleus and its formation is inhibited by RPA-2. While RPA-4 does not participate in replication or recombination, we find that RPA-4 inhibits RAD-51 filament formation and promotes apoptosis of a subset of damaged nuclei. Altogether these findings point to sub-functionalization and antagonistic roles of RPA complexes in C. elegans.
Details
- Title: Subtitle
- RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis
- Creators
- Adam Hefel - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAMasayoshi Honda - Department of Biochemistry, The University of Iowa Carver College of Medicine, Iowa City, IA 52242, USANicholas Cronin - Department of Biochemistry, The University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAKailey Harrell - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAPooja Patel - Department of Biology, The University of Iowa, Iowa City, IA 52242, USAMaria Spies - Department of Biochemistry, The University of Iowa Carver College of Medicine, Iowa City, IA 52242, USASarit Smolikove - Department of Biology, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.49(4), pp.2005-2026
- DOI
- 10.1093/nar/gkaa1293
- PMID
- 33476370
- PMCID
- PMC7913698
- NLM abbreviation
- Nucleic Acids Res
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Grant note
- R01 GM112657 / NIGMS NIH HHS R35 GM131704 / NIGMS NIH HHS P40 OD010440 / NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 02/26/2021
- Academic Unit
- Biology; Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984217413102771
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