RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Yukihide Maeda; Kunihiro Fukushima; Masashi Kakiuchi; Yorihisa Orita; Kazunori Nishizaki; Richard J H Smith

doi:10.1097/00001756-200503150-00011

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RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Journal article

Peer reviewed

RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Yukihide Maeda, Kunihiro Fukushima, Masashi Kakiuchi, Yorihisa Orita, Kazunori Nishizaki and Richard J H Smith

Neuroreport, Vol.16(4), pp.361-365

03/15/2005

DOI: 10.1097/00001756-200503150-00011

PMID: 15729138

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Abstract

Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.

Extracellular Matrix Proteins - biosynthesis

Extracellular Matrix Proteins - genetics

Membrane Glycoproteins - biosynthesis

GPI-Linked Proteins

Membrane Glycoproteins - genetics

RNA - genetics

Proteins - genetics

RNA - biosynthesis

Animals

Proteins - metabolism

Cochlea - metabolism

Reverse Transcriptase Polymerase Chain Reaction - methods

Trans-Activators - genetics

Fetus

Mice

Mice, Inbred BALB C

Trans-Activators - biosynthesis

Eye Proteins - genetics

Organ Culture Techniques

Eye Proteins - biosynthesis

Details

Title: Subtitle: RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants
Creators: Yukihide Maeda - Molecular Otolaryngology Research Laboratory, Department of Otolaryngology, The University of Iowa Hospitals and Clinics, Iowa City, IA 52242, USA
Kunihiro Fukushima
Masashi Kakiuchi
Yorihisa Orita
Kazunori Nishizaki
Richard J H Smith
Resource Type: Journal article
Publication Details: Neuroreport, Vol.16(4), pp.361-365
DOI: 10.1097/00001756-200503150-00011
PMID: 15729138
NLM abbreviation: Neuroreport
ISSN: 0959-4965
eISSN: 1473-558X
Publisher: England
Grant note: R01-DC02842 / NIDCD NIH HHS
Language: English
Date published: 03/15/2005
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Otolaryngology; Internal Medicine
Record Identifier: 9984006456002771

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