Rac1 is required for cell proliferation and G2/M progression

Kimberly A MOORE; Rachna SETHI; A. Masharn DOANES; Thomas M JOHNSON; John B PRACYK; Martha KIRBY; Kaikobad IRANI; Pascal J GOLDSCHMIDT-CLERMONT; Toren FINKEL

doi:10.1042/bj3260017

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Rac1 is required for cell proliferation and G2/M progression

Journal article

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Peer reviewed

Rac1 is required for cell proliferation and G2/M progression

Kimberly A MOORE, Rachna SETHI, A. Masharn DOANES, Thomas M JOHNSON, John B PRACYK, Martha KIRBY, Kaikobad IRANI, Pascal J GOLDSCHMIDT-CLERMONT and Toren FINKEL

Biochemical journal, Vol.326(1), pp.17-20

08/15/1997

DOI: 10.1042/bj3260017

PMCID: PMC1218631

PMID: 9337845

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Abstract

We have transiently expressed a dominant negative form of rac1 (N17rac1) using adenoviral-mediated gene transfer. The level of N17rac1 expression is demonstrated to be proportional to the multiplicity of infection. Expression of N17rac1 in Rat 2 fibroblasts results in cytostatic growth arrest. Cell-cycle analysis demonstrates that cells expressing N17rac1 accumulate in G2/M. These results suggest that rac1 is required for cell proliferation and provide the first demonstration in mammalian cells of a role for small GTP-binding proteins in the G2/M transition.

Details

Title: Subtitle: Rac1 is required for cell proliferation and G2/M progression
Creators: Kimberly A MOORE - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
Rachna SETHI - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
A. Masharn DOANES - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
Thomas M JOHNSON - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
John B PRACYK - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
Martha KIRBY - Hematology Branch, NHLBI, NIH, Bethesda, MD 20892, U.S.A
Kaikobad IRANI - Cardiology Division, Johns Hopkins Hospital, Baltimore, MD 21287, U.S.A
Pascal J GOLDSCHMIDT-CLERMONT - Heart and Lung Institute, Ohio State University, Columbus, OH 43210, U.S.A
Toren FINKEL - Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20892-1650, U.S.A
Resource Type: Journal article
Publication Details: Biochemical journal, Vol.326(1), pp.17-20
DOI: 10.1042/bj3260017
PMID: 9337845
PMCID: PMC1218631
ISSN: 0264-6021
eISSN: 1470-8728
Language: English
Date published: 08/15/1997
Academic Unit: Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984047713202771

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