Radio frequency glow discharge-induced acidification of fluoropolymers

Benjamin M Krawczyk; Jonas Baltrusaitis; Colin M Yoder; Terrence G Vargo; Ned B Bowden; Khalid N Kader

doi:10.1002/jbm.a.33173

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Radio frequency glow discharge-induced acidification of fluoropolymers

Journal article

Peer reviewed

Radio frequency glow discharge-induced acidification of fluoropolymers

Benjamin M Krawczyk, Jonas Baltrusaitis, Colin M Yoder, Terrence G Vargo, Ned B Bowden and Khalid N Kader

Journal of biomedical materials research. Part A, Vol.99(3), pp.418-425

12/01/2011

DOI: 10.1002/jbm.a.33173

PMID: 21887736

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Abstract

Fluoropolymer surfaces are unique in view of the fact that they are quite inert, have low surface energies, and possess high thermal stabilities. Attempts to modify fluoropolymer surfaces have met with difficulties in that it is difficult to control the modification to maintain bulk characteristics of the polymer. In a previously described method, the replacement of a small fraction of surface fluorine by acid groups through radio frequency glow discharge created a surface with unexpected reactivity allowing for attachment of proteins in their active states. The present study demonstrates that 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) reacts with the acid groups on fluoropolymer surfaces in a novel reaction not previously described. This reaction yields an excellent leaving group in which a primary amine on proteins can substitute to form a covalent bond between a protein and these surfaces. In an earlier study, we demonstrated that collagen IV could be deposited on a modified PTFE surface using EDC as a linker. Once collagen IV is attached to the surface, it assembles to form a functional stratum resembling collagen IV in native basement membrane. In this study, we show data suggesting that the fluorine to carbon ratio determines the acidity of the fluoropolymer surfaces and how well collagen IV attaches to and assembles on four different fluoropolymer surfaces.

Collagen Type IV - metabolism

Fluorocarbons - chemistry

Photoelectron Spectroscopy

Acids - chemistry

Humans

Radio Waves

Polymerization

Polytetrafluoroethylene - chemistry

Fluorine - analysis

Thermodynamics

Surface Properties

Polymers - chemistry

Carbon - analysis

Polyvinyls - chemistry

Details

Title: Subtitle: Radio frequency glow discharge-induced acidification of fluoropolymers
Creators: Benjamin M Krawczyk - Cell and Synthetic Interface Engineering Laboratory, Department of Biomedical Engineering, University of Iowa, Iowa City, Iowa 52242, USA
Jonas Baltrusaitis
Colin M Yoder
Terrence G Vargo
Ned B Bowden
Khalid N Kader
Resource Type: Journal article
Publication Details: Journal of biomedical materials research. Part A, Vol.99(3), pp.418-425
Publisher: United States
DOI: 10.1002/jbm.a.33173
PMID: 21887736
ISSN: 1549-3296
eISSN: 1552-4965
Language: English
Date published: 12/01/2011
Academic Unit: Chemistry
Record Identifier: 9983985979702771

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