Radioimmunoassay for the calcium release channel agonist ryanodine

Steven D Kahl; Peter S Mcpherson; Terence Lewis; Philip Bentley; Michael J Mullinnix; John D Windass; Kevin P Campbell

doi:10.1006/abio.1994.1140

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Radioimmunoassay for the calcium release channel agonist ryanodine

Journal article

Peer reviewed

Radioimmunoassay for the calcium release channel agonist ryanodine

Steven D Kahl, Peter S Mcpherson, Terence Lewis, Philip Bentley, Michael J Mullinnix, John D Windass and Kevin P Campbell

Analytical biochemistry, Vol.218(1), pp.55-62

1994

DOI: 10.1006/abio.1994.1140

PMID: 8053568

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Abstract

A novel photo-activatable derivative of ryanodine, 9-hydroxy-21-(4-azidobenzoyloxy)-9-epiryanodine, has been synthesized and conjugated to keyhole limpet hemocyanin for the production of antibodies with high affinity and specificity to ryanodine. The anti-ryanodine antibodies reacted specifically on immunoblots with the azido-ryanodine compound covalently conjugated to bovine serum albumin. A radioimmunoassay specific for ryanodine was developed using the anti-ryanodine antibodies, and a dissociation constant for ryanodine of 1 nM was determined. Half-maximal inhibition constants (IC50) for various ryanodine derivatives were found to range between 3.2 and 200 nM. These IC50 values correlated very well with the IC50 values obtained for the compounds binding to the skeletal muscle membrane receptor. These antibodies should be useful for the characterization of the ryanodine binding site on the sarcoplasmic reticulum Ca2+ release channel.

Fundamental and applied biological sciences. Psychology

Alcaloids

Biological and medical sciences

Analytical, structural and metabolic biochemistry

Heterocyclic compounds, pigments

Other biological molecules

Details

Title: Subtitle: Radioimmunoassay for the calcium release channel agonist ryanodine
Creators: Steven D Kahl - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Peter S Mcpherson - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Terence Lewis - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Philip Bentley
Michael J Mullinnix - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
John D Windass
Kevin P Campbell - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: Analytical biochemistry, Vol.218(1), pp.55-62
Publisher: Elsevier; San Diego, CA
DOI: 10.1006/abio.1994.1140
PMID: 8053568
ISSN: 0003-2697
eISSN: 1096-0309
Language: English
Date published: 1994
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984020707502771

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