Journal article
Radiolabeled, Antibody-Conjugated Manganese Oxide Nanoparticles for Tumor Vasculature Targeted Positron Emission Tomography and Magnetic Resonance Imaging
ACS applied materials & interfaces, Vol.9(44), pp.38304-38312
11/08/2017
DOI: 10.1021/acsami.7b12216
PMCID: PMC5680099
PMID: 29028311
Abstract
Manganese oxide nanoparticles (Mn3O4 NPs) have attracted a great deal of attention in the field of biomedical imaging because of their ability to create an enhanced imaging signal in MRI as novel potent T-1 contrast agents. In this study, we present tumor vasculature-targeted imaging in mice using Mn3O4 NPs through conjugation to the anti-CD105 antibody TRC105 and radionuclide copper-64 (Cu-64, t(1/2): 12.7 h). The Mn3O4 conjugated NPs, Cu-64-NOTAMn(3)O(4)@PEG-TRC105, exhibited sufficient stability in vitro and in vivo. Serial positron emission tomography (PET) and magnetic resonance imaging (MRI) studies evaluated the pharmacokinetics and demonstrated targeting of Cu-64-NOTA-Mn3O4@PEG-TRC105 to 4T(1) murine breast tumors in vivo, compared to Cu-64-NOTA-Mn3O4@PEG. The specificity of Cu-64-NOTA-Mn3O4@PEG-TRC105 for the vascular marker CD105 was confirmed through in vivo, in vitro, and ex vivo experiments. Since Mn3O4 conjugated NPs exhibited desirable properties for T1 enhanced imaging and low toxicity, the tumor-specific Mn3O4 conjugated NPs reported in this study may serve as promising multifunctional nanoplatforms for precise cancer imaging and diagnosis.
Details
- Title: Subtitle
- Radiolabeled, Antibody-Conjugated Manganese Oxide Nanoparticles for Tumor Vasculature Targeted Positron Emission Tomography and Magnetic Resonance Imaging
- Creators
- Yonghua Zhan - Xidian UniversitySixiang Shi - University of Wisconsin–MadisonEmily B. Ehlerding - University of Wisconsin–MadisonStephen A. Graves - University of Wisconsin–MadisonShreya Goel - Univ Wisconsin, Dept Radiol, Madison, WI 53705 USAJonathan W. Engle - University of Wisconsin–MadisonJimin Liang - Xidian UniversityJie Tian - Institute of Automation, Chinese Academy of Sciences Beijing, 100190, ChinaWeibo Cai - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- ACS applied materials & interfaces, Vol.9(44), pp.38304-38312
- DOI
- 10.1021/acsami.7b12216
- PMID
- 29028311
- PMCID
- PMC5680099
- NLM abbreviation
- ACS Appl Mater Interfaces
- ISSN
- 1944-8244
- eISSN
- 1944-8252
- Publisher
- Amer Chemical Soc
- Number of pages
- 9
- Grant note
- R01EB021336 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) 81227901; 11727813; 81571725; 81230033; 31371006; 61405149; 81660505; 81627807 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) P30CA014520 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) JB171204 / Fundamental Research Funds for the Central Universities NIBIB/NCI 1R01CA169365; 1R01CA205101; 1R01EB021336; T32GM008505; T32CA009206; P30CA014520 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA University of Wisconsin Madison 125246-RSG-13-099-01-CCE / American Cancer Society T32GM008505 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 2017JM8057 / Natural Science Basic Research Plan in Shaanxi Province of China
- Language
- English
- Date published
- 11/08/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Radiation Oncology
- Record Identifier
- 9984383889102771
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