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Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission
Journal article   Open access   Peer reviewed

Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission

Li Wu, Thomas D Martin, Mary Carrington and Vineet N KewalRamani
Virology (New York, N.Y.), Vol.318(1), pp.17-23
01/05/2004
DOI: 10.1016/j.virol.2003.09.028
PMID: 14972530
url
https://doi.org/10.1016/j.virol.2003.09.028View
Published (Version of record) Open Access

Abstract

A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.
Cell Line Lectins, C-Type - metabolism Monocytes - virology Cell Adhesion Molecules - genetics HIV-1 - pathogenicity Humans B-Lymphocytes - virology Receptors, Cell Surface - metabolism HIV Infections - transmission Lectins, C-Type - genetics Cell Adhesion Molecules - metabolism Receptors, Cell Surface - genetics

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