RalA and RalB differentially regulate development of epithelial tight junctions

C. Clayton Hazelett; David Sheff; Charles Yeaman

doi:10.1091/mbc.e11-07-0657

Back

RalA and RalB differentially regulate development of epithelial tight junctions

Journal article

Open access

Peer reviewed

RalA and RalB differentially regulate development of epithelial tight junctions

C. Clayton Hazelett, David Sheff and Charles Yeaman

Molecular biology of the cell, Vol.22(24), pp.4787-4800

12/15/2011

DOI: 10.1091/mbc.e11-07-0657

PMCID: PMC3237622

PMID: 22013078

Files and links (1)

url

https://doi.org/10.1091/mbc.e11-07-0657View

Published (Version of record) Open Access

Abstract

Tight junctions (TJs) are structures indispensable to epithelial cells and are responsible for regulation of paracellular diffusion and maintenance of cellular polarity. Although many interactions between TJ constituents have been identified, questions remain concerning how specific functions of TJs are established and regulated. Here we investigated the roles of Ral GTPases and their common effector exocyst complex in the formation of nascent TJs. Unexpectedly, RNA interference–mediated suppression of RalA or RalB caused opposing changes in TJ development. RalA reduction increased paracellular permeability and decreased incorporation of components into TJs, whereas RalB reduction decreased paracellular permeability and increased incorporation of components into TJs. Activities of both Ral GTPases were mediated through the exocyst. Finally, we show that TJ-mediated separation of apical–basal membrane domains is established prior to equilibration of barrier function and that it is unaffected by Ral knockdown or specific composition of TJs.

Details

Title: Subtitle: RalA and RalB differentially regulate development of epithelial tight junctions
Creators: C. Clayton Hazelett - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242
David Sheff - Department of Pharmacology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242
Charles Yeaman - Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242
Contributors: Asma Nusrat (Editor)
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.22(24), pp.4787-4800
DOI: 10.1091/mbc.e11-07-0657
PMID: 22013078
PMCID: PMC3237622
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology
Language: English
Date published: 12/15/2011
Academic Unit: Anatomy and Cell Biology; Family and Community Medicine; Internal Medicine
Record Identifier: 9984025336002771

Metrics

26 Record Views

26 Times Cited - Web of Science