RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase

Peter Askjaer; Angela Bachi; Matthias Wilm; F. Ralf Bischoff; Daniel L Weeks; Vera Ogniewski; Mutsuhito Ohno; Christof Niehrs; Jørgen Kjems; Iain W Mattaj; Maarten Fornerod

doi:10.1128/MCB.19.9.6276

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RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase

Journal article

Peer reviewed

RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase

Peter Askjaer, Angela Bachi, Matthias Wilm, F. Ralf Bischoff, Daniel L Weeks, Vera Ogniewski, Mutsuhito Ohno, Christof Niehrs, Jørgen Kjems, Iain W Mattaj, …

Molecular and cellular biology, Vol.19(9), pp.6276-6285

09/1999

DOI: 10.1128/MCB.19.9.6276

PMCID: PMC84588

PMID: 10454574

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Abstract

CRM1 is an export receptor mediating rapid nuclear exit of proteins and RNAs to the cytoplasm. CRM1 export cargoes include proteins with a leucine-rich nuclear export signal (NES) that bind directly to CRM1 in a trimeric complex with RanGTP. Using a quantitative CRM1-NES cargo binding assay, significant differences in affinity for CRM1 among natural NESs are demonstrated, suggesting that the steady-state nucleocytoplasmic distribution of shuttling proteins could be determined by the relative strengths of their NESs. We also show that a trimeric CRM1-NES-RanGTP complex is disassembled by RanBP1 in the presence of RanGAP, even though RanBP1 itself contains a leucine-rich NES. Selection of CRM1-binding proteins from Xenopus egg extract leads to the identification of an NES-containing DEAD-box helicase, An3, that continuously shuttles between the nucleus and the cytoplasm. In addition, we identify the Xenopus homologue of the nucleoporin CAN/Nup214 as a RanGTP- and NES cargo-specific binding site for CRM1, suggesting that this nucleoporin plays a role in export complex disassembly and/or CRM1 recycling.

Gene Expression

Details

Title: Subtitle: RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase
Creators: Peter Askjaer - Departments of Gene Expression
Angela Bachi - Departments of Gene Expression
Matthias Wilm - Departments of Gene Expression
F. Ralf Bischoff - Departments of Gene Expression
Daniel L Weeks - Departments of Gene Expression
Vera Ogniewski - Departments of Gene Expression
Mutsuhito Ohno - Departments of Gene Expression
Christof Niehrs - Departments of Gene Expression
Jørgen Kjems - Departments of Gene Expression
Iain W Mattaj - Departments of Gene Expression
Maarten Fornerod - Departments of Gene Expression
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.19(9), pp.6276-6285
DOI: 10.1128/MCB.19.9.6276
PMID: 10454574
PMCID: PMC84588
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: American Society for Microbiology
Language: English
Date published: 09/1999
Academic Unit: Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
Record Identifier: 9984025287202771

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