Journal article
Rapid, Culture-Free Detection of Staphylococcus aureus Bacteremia
PloS one, Vol.11(6), pp.e0157234-e0157234
2016
DOI: 10.1371/journal.pone.0157234
PMCID: PMC4909304
PMID: 27305148
Abstract
S. aureus bacteremia (SAB) is a common condition with high rates of morbidity and mortality. Current methods used to diagnose SAB take at least a day, and often longer. Patients with suspected bacteremia must therefore be empirically treated, often unnecessarily, while assay results are pending. In this proof-of-concept study, we describe an inexpensive assay that detects SAB via the detection of micrococcal nuclease (an enzyme secreted by S. aureus) in patient plasma samples in less than three hours. In total, 17 patient plasma samples from culture-confirmed S. aureus bacteremic individuals were tested. 16 of these yielded greater nuclease assay signals than samples from uninfected controls or individuals with non-S. aureus bacteremia. These results suggest that a nuclease-detecting assay may enable the rapid and inexpensive diagnosis of SAB, which is expected to substantially reduce the mortality and morbidity that result from this condition.
Details
- Title: Subtitle
- Rapid, Culture-Free Detection of Staphylococcus aureus Bacteremia
- Creators
- Elliot L Burghardt - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaKatie S Flenker - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaKaren C Clark - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaJeff Miguel - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaDilek Ince - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaPatricia Winokur - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaBradley Ford - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of AmericaJames O McNamara II - Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, United States of America
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.11(6), pp.e0157234-e0157234
- DOI
- 10.1371/journal.pone.0157234
- PMID
- 27305148
- PMCID
- PMC4909304
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; United States
- Grant note
- R21 AI101391 / NIAID NIH HHS I01 BX000543 / BLRD VA U54 TR001356 / NCATS NIH HHS T32 GM007337 / NIGMS NIH HHS R01 AI106738 / NIAID NIH HHS P30 DK054759 / NIDDK NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 2016
- Academic Unit
- Infectious Diseases; Pathology; Medicine Administration; Internal Medicine
- Record Identifier
- 9984046831002771
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