Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

Reid A Simmer; Patrick M Richards; Jessica M Ewald; Cory Schwarz; Marcio L. B da Silva; Jacques Mathieu; Pedro J. J Alvarez; Jerald L Schnoor

doi:10.1021/acs.estlett.1c00714

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Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

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Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

Reid A Simmer, Patrick M Richards, Jessica M Ewald, Cory Schwarz, Marcio L. B da Silva, Jacques Mathieu, Pedro J. J Alvarez and Jerald L Schnoor

Environmental science & technology letters, Vol.8(11), pp.975-980

10/04/2021

DOI: 10.1021/acs.estlett.1c00714

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Abstract

Bioremediation is a promising treatment technology for 1,4-dioxane-contaminated groundwater. However, metabolic dioxane-degrading bacteria identified to date are limited by their slow kinetics and inability to sustain growth at low dioxane concentrations (<100 μg/L). Furthermore, strains may underperform because of missing growth factors, such as amino acids or vitamins. In this work, we reevaluate Rhodococcus ruber strain 219 as a dioxane-degrading strain with bioaugmentation potential. We report rapid growth and metabolic dioxane degradation by R. ruber 219 when supplemented with thiamine (vitamin B1). We also discern that the strain lacks a complete de novo thiamine synthesis pathway, indicating that R. ruber 219 is a probable thiamine auxotroph. However, when supplemented with thiamine, the strain’s Monod kinetics (Ks = 0.015 ± 0.03 μg/L) and exceedingly low Smin (0.49 ± 1.16 μg/L) suggest this strain can maintain growth at very low dioxane concentrations (<100 μg/L). Accordingly, we demonstrate that thiamine-grown R. ruber 219 sustains degradation of dilute dioxane (<100 μg/L) to below health advisory levels. This is the first study to report sustained metabolic dioxane biodegradation to below health advisory levels of 0.35 μg/L. Overall, our findings solidify R. ruber 219 as a promising candidate for bioremediation of dioxane-contaminated groundwater.

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Title: Subtitle: Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219
Creators: Reid A Simmer - University of Iowa
Patrick M Richards - University of Iowa
Jessica M Ewald - University of Iowa
Cory Schwarz - Rice University
Marcio L. B da Silva - Rice University
Jacques Mathieu - Rice University
Pedro J. J Alvarez - Rice University
Jerald L Schnoor - University of Iowa
Resource Type: Journal article
Publication Details: Environmental science & technology letters, Vol.8(11), pp.975-980
DOI: 10.1021/acs.estlett.1c00714
ISSN: 2328-8930
eISSN: 2328-8930
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100013316, name: Strategic Environmental Research and Development Program, award: ER-2719; name: W. M. Keck Phytotechnologies Laboratory, University of Iowa
Language: English
Date published: 10/04/2021
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; IIHR--Hydroscience and Engineering
Record Identifier: 9984186940102771

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