Journal article
Rapid Screening of Mineralization Capacity of Biomaterials by Means of Quantification of Enzymatically Deposited Calcium Phosphate
Tissue engineering. Part C, Methods, Vol.20(10), pp.838-850
10/01/2014
DOI: 10.1089/ten.tec.2013.0334
PMID: 24650236
Abstract
The current study focused on the development of a rapid, straightforward quantification method based on the use of enzymatic decomposition of urea using urease to assess the mineralization capacity of a wide range of biomaterials for bone regeneration. Urea-containing mineralizing solutions (MSs) (containing: Na2HPO4, CaCl2, and NaCl at 37 degrees C and pH 6.0) were used in the mineralization experiments. Urease was added to these solutions to induce enzymatic decomposition of urea resulting in increased pH and deposition of calcium phosphate. By optimizing the ionic and urease concentrations in these MSs, it was shown that the proposed system could mineralize titanium substrates with six different pretreatments, as opposed to normal simulated body fluid that mineralized only two of them. It was possible to rank the mineralization capacity of these substrates by measuring the amount of calcium deposited. Furthermore, the ranking of (i) various polymeric substrates and (ii) hydrogels with and without functionalization with calcium-binding bisphosphonate groups was also possible. These results confirm that the proposed testing system has a broad applicability in the field of biomaterials due to its inherent versatility and discriminative power.
Details
- Title: Subtitle
- Rapid Screening of Mineralization Capacity of Biomaterials by Means of Quantification of Enzymatically Deposited Calcium Phosphate
- Creators
- Arnold W. G. Nijhuis - Radboud University Medical CenterShinji TakemotoM. Reza NejadnikYubao LiXia Yang - PolymerkemiDmitri A. Ossipov - PolymerkemiJöns Hilborn - PolymerkemiAntonios G. MikosMasao YoshinariJohn A. JansenSander C. G. Leeuwenburgh
- Resource Type
- Journal article
- Publication Details
- Tissue engineering. Part C, Methods, Vol.20(10), pp.838-850
- DOI
- 10.1089/ten.tec.2013.0334
- PMID
- 24650236
- ISSN
- 1937-3392
- eISSN
- 1937-3392
- Language
- English
- Date published
- 10/01/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984421974202771
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