Journal article
Rapid Volumetric Bioprinting of Decellularized Extracellular Matrix Bioinks
Advanced materials (Weinheim), Vol.36(34), e2304846
01/22/2024
DOI: 10.1002/adma.202304846
PMCID: PMC11260906
PMID: 38252896
Abstract
Decellularized extracellular matrix (dECM)‐based hydrogels are widely applied to additive biomanufacturing strategies for relevant applications. The extracellular matrix components and growth factors of dECM play a crucial role in cell adhesion, growth, and differentiation. However, the generally poor mechanical properties and printability have remained as major limitations for dECM‐based materials. In this study, heart‐derived dECM (h‐dECM) and meniscus‐derived dECM (Ms‐dECM) bioinks in their pristine, unmodified state supplemented with the photoinitiator of tris(2,2‐bipyridyl) dichlororuthenium(II) hexahydrate and sodium persulfate, demonstrate cytocompatibility with volumetric bioprinting processes. This recently developed bioprinting modality illuminates a dynamically evolving light pattern into a rotating volume of the bioink, and thus decouples the requirement of mechanical strengths of bioprinted hydrogel constructs with printability, allowing for the fabrication of sophisticated shapes and architectures with low‐concentration dECM materials that set within tens of seconds. As exemplary applications, cardiac tissues are volumetrically bioprinted using the cardiomyocyte‐laden h‐dECM bioink showing favorable cell proliferation, expansion, spreading, biomarker expression, and synchronized contractions; whereas the volumetrically bioprinted Ms‐dECM meniscus structures embedded with human mesenchymal stem cells present appropriate chondrogenic differentiation outcomes. This study supplies expanded bioink libraries for volumetric bioprinting and broadens utilities of dECM towards tissue engineering and regenerative medicine. This article is protected by copyright. All rights reserved
Details
- Title: Subtitle
- Rapid Volumetric Bioprinting of Decellularized Extracellular Matrix Bioinks
- Creators
- Liming Lian - Brigham and Women's HospitalMaobin Xie - Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge MA 02139 USAZeyu Luo - Brigham and Women's HospitalZhenrui Zhang - Brigham and Women's HospitalSushila Maharjan - Brigham and Women's HospitalXuan Mu (Author) - University of Iowa, Roy J. Carver Department of Biomedical EngineeringCarlos Ezio Garciamendez-Mijares - Brigham and Women's HospitalXiao Kuang - Harvard UniversityJugal Kishore Sahoo - Tufts UniversityGuosheng Tang - Harvard UniversityGang Li - Tufts UniversityDi Wang - Brigham and Women's HospitalJie Guo - Harvard UniversityFederico Zertuche González - Brigham and Women's HospitalVictoria Manjarrez - Brigham and Women's HospitalLing Cai - Brigham and Women's HospitalXuan Mei - Brigham and Women's HospitalDavid L. Kaplan - Tufts UniversityYu Shrike Zhang - Brigham and Women's Hospital
- Resource Type
- Journal article
- Publication Details
- Advanced materials (Weinheim), Vol.36(34), e2304846
- DOI
- 10.1002/adma.202304846
- PMID
- 38252896
- PMCID
- PMC11260906
- NLM abbreviation
- Adv Mater
- ISSN
- 0935-9648
- eISSN
- 1521-4095
- Grant note
- This work was performed in part at the Center for Nanoscale Systems (CNS), Harvard University, and supported by the National Institutes of Health (P41EB027062, R01AR070975, R01EB028143, R01HL165176, R01HL166522, R01CA282451, R21EB030257), the National Science Foundation award (1541959, CBET-EBMS-1936105, CISE-IIS-2225698), the ARO (W911NF2120130), the AFOSR (FA9550-20-1-0363), the Chan Zuckerberg Initiative (2022-316712), and the Brigham Research Institute.
- Language
- English
- Electronic publication date
- 01/22/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984558245102771
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