Rapid formation and remodeling of postsynaptic densities in developing dendrites

G S Marrs; S H Green; M E Dailey

doi:10.1038/nn717

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Rapid formation and remodeling of postsynaptic densities in developing dendrites

Journal article

Peer reviewed

Rapid formation and remodeling of postsynaptic densities in developing dendrites

G S Marrs, S H Green and M E Dailey

Nature neuroscience, Vol.4(10), pp.1006-1013

10/2001

DOI: 10.1038/nn717

PMID: 11574832

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Abstract

The dynamics of postsynaptic density (PSD) formation and remodeling were investigated in live developing hippocampal tissue slices. Time lapse imaging of transfected neurons expressing GFP-tagged PSD95, a prominent PSD protein, revealed that up to 40% of PSDs in developing dendrites are structurally dynamic; they rapidly (<15 min) appear or disappear, but also grow, shrink and move within shafts and spines. New spines containing PSDs were formed by conversion of dynamic filopodia-like spine precursors in which PSDs appeared de novo, or by direct extension of spines or spine precursors carrying preformed PSDs from the shaft. PSDs are therefore highly dynamic structures that can undergo rapid structural alteration within dendrite shafts, spines and spine precursors, permitting rapid formation and remodeling of synaptic connections in developing CNS tissues.

Green Fluorescent Proteins

Cell Surface Extensions - ultrastructure

Cell Surface Extensions - metabolism

Culture Techniques

Rats

Microscopy, Video

Nerve Tissue Proteins - genetics

Recombinant Fusion Proteins - metabolism

Synapsins - metabolism

Nerve Tissue Proteins - metabolism

Microscopy, Confocal

Animals

Synapses - metabolism

Image Processing, Computer-Assisted

Time Factors

Dendrites - physiology

Hippocampus - ultrastructure

Hippocampus - physiology

Genes, Reporter

Luminescent Proteins - metabolism

Details

Title: Subtitle: Rapid formation and remodeling of postsynaptic densities in developing dendrites
Creators: G S Marrs - Department of Biological Sciences, 335 Biology Building, University of Iowa, Iowa City, Iowa 52242, USA
S H Green
M E Dailey
Resource Type: Journal article
Publication Details: Nature neuroscience, Vol.4(10), pp.1006-1013
Publisher: United States
DOI: 10.1038/nn717
PMID: 11574832
ISSN: 1097-6256
eISSN: 1546-1726
Grant note: R29 NS037159 / NINDS NIH HHS NS37159 / NINDS NIH HHS DC02961 / NIDCD NIH HHS R01 DC002961 / NIDCD NIH HHS
Language: English
Date published: 10/2001
Academic Unit: Iowa Neuroscience Institute; Biology; Otolaryngology
Record Identifier: 9983991988602771

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