Journal article
Rapid method to screen and sort lipid accumulating microalgae
Bioresource technology, Vol.184, pp.47-52
05/01/2015
DOI: 10.1016/j.biortech.2014.10.057
PMID: 25453436
Abstract
•Optimized method for detecting lipid rich cells of C. littorale with FACS.•Efficient BODIPY staining dissolved only in ethanol, no assistant necessary.•Maintenance of cellular viability after staining and cell sorting.•The process can be applied to sort cells with high lipid content.
The present work established an efficient staining method for fluorescence activated cell sorting (FACS) with Chlorococcum littorale maintaining cellular viability. The method was designed to detect high-lipid cells and to guarantee cellular viability. BODIPY505/515 (BP) was more suitable to FACS when compared to Nile red. The optimum concentrations were 0.4μgml−1 of BP, 0.1% DMSO or 0.35% ethanol. Both ethanol and DMSO were equally efficient and assured cellular viability after the staining and sorting. Here a method is presented to rapidly screen and sort lipid rich cells of C. littorale with FACS, which can be used to produce new inoculum with increased cellular lipid content.
Details
- Title: Subtitle
- Rapid method to screen and sort lipid accumulating microalgae
- Creators
- Iago Teles Dominguez Cabanelas - Wageningen University & ResearchMathijs van der Zwart - Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8128, 6700 EV Wageningen, NetherlandsDorinde M.M. Kleinegris - Wageningen University & ResearchMaria J. Barbosa - Wageningen University and Research Center, Food and Biobased Research, AlgaePARC, Bornsesteeg 10, Building 112, 6721NG Bennekom, NetherlandsRené H. Wijffels - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Bioresource technology, Vol.184, pp.47-52
- DOI
- 10.1016/j.biortech.2014.10.057
- PMID
- 25453436
- NLM abbreviation
- Bioresour Technol
- ISSN
- 0960-8524
- eISSN
- 1873-2976
- Publisher
- Elsevier Ltd
- Grant note
- name: Ministry of Economic Affairs, Agriculture and Innovation; name: Province of Gelderland; name: National Council of Science and Technology, award: 236614/2012-6
- Language
- English
- Date published
- 05/01/2015
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398012302771
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