Rat Calvarial Bone Regeneration by 3D-Printed β‑Tricalcium Phosphate Incorporating MicroRNA-200c

Matthew T Remy; Adil Akkouch; Li He; Steven Eliason; Mason E Sweat; Tadkamol Krongbaramee; Fan Fei; Fang Qian; Brad A Amendt; Xuan Song; Liu Hong

doi:10.1021/acsbiomaterials.0c01756

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Rat Calvarial Bone Regeneration by 3D-Printed β‑Tricalcium Phosphate Incorporating MicroRNA-200c

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Rat Calvarial Bone Regeneration by 3D-Printed β‑Tricalcium Phosphate Incorporating MicroRNA-200c

Matthew T Remy, Adil Akkouch, Li He, Steven Eliason, Mason E Sweat, Tadkamol Krongbaramee, Fan Fei, Fang Qian, Brad A Amendt, Xuan Song, …

ACS biomaterials science & engineering, Vol.7(9), pp.4521-4534

09/13/2021

DOI: 10.1021/acsbiomaterials.0c01756

PMID: 34437807

Appears in UI Libraries Support Open Access

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Abstract

Advanced fabrication methods for bone grafts designed to match defect sites that combine biodegradable, osteoconductive materials with potent, osteoinductive biologics would significantly impact the clinical treatment of large bone defects. In this study, we engineered synthetic bone grafts using a hybrid approach that combined three-dimensional (3D-)printed biodegradable, osteoconductive β-tricalcium phosphate (β-TCP) with osteoinductive microRNA(miR)-200c. 3D-printed β-TCP scaffolds were fabricated utilizing a suspension-enclosing projection-stereolithography (SEPS) process to produce constructs with reproducible microarchitectures that enhanced the osteoconductive properties of β-TCP. Collagen coating on 3D-printed β-TCP scaffolds slowed the release of plasmid DNA encoding miR-200c compared to noncoated constructs. 3D-printed β-TCP scaffolds coated with miR-200c-incorporated collagen increased the transfection efficiency of miR-200c of both rat and human BMSCs and additionally increased osteogenic differentiation of hBMSCs in vitro. Furthermore, miR-200c-incorporated scaffolds significantly enhanced bone regeneration in critical-sized rat calvarial defects. These results strongly indicate that bone grafts combining SEPS 3D-printed osteoconductive biomaterial-based scaffolds with osteoinductive miR-200c can be used as superior bone substitutes for the clinical treatment of large bone defects.

Tissue Engineering and Regenerative Medicine

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Title: Subtitle: Rat Calvarial Bone Regeneration by 3D-Printed β‑Tricalcium Phosphate Incorporating MicroRNA-200c
Creators: Matthew T Remy - University of Iowa
Adil Akkouch - University of Iowa
Li He - University of Iowa
Steven Eliason - University of Iowa
Mason E Sweat - University of Iowa
Tadkamol Krongbaramee - University of Iowa
Fan Fei - University of Iowa
Fang Qian - University of Iowa
Brad A Amendt - University of Iowa
Xuan Song - University of Iowa
Liu Hong - University of Iowa
Resource Type: Journal article
Publication Details: ACS biomaterials science & engineering, Vol.7(9), pp.4521-4534
DOI: 10.1021/acsbiomaterials.0c01756
PMID: 34437807
NLM abbreviation: ACS Biomater Sci Eng
ISSN: 2373-9878
eISSN: 2373-9878
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE026433, R03EB025873, R21DE024799, T90DE023520; DOI: 10.13039/100000147, name: Division of Civil, Mechanical and Manufacturing Innovation, award: CMMI-1825962
Language: English
Date published: 09/13/2021
Academic Unit: Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Prosthodontics; Industrial and Systems Engineering; Craniofacial Anomalies Research Center; Injury Prevention Research Center; Dental Research; Mechanical Engineering
Record Identifier: 9984186959302771

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