Rat NKR‐P1+CD3+T cells: selective proliferation in interleukin‐2, diverse T‐cell‐receptor‐Vβ repertoire and polarized interferon‐γ expression

V Badovinac; C Boggiano; V Trajković; A B Frey; N L Vujanović; D P Gold; M Mostarica-Stojković; S Vukmanović

doi:10.1046/j.1365-2567.1998.00567.x

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Rat NKR‐P1+CD3+T cells: selective proliferation in interleukin‐2, diverse T‐cell‐receptor‐Vβ repertoire and polarized interferon‐γ expression

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Rat NKR‐P1+CD3+T cells: selective proliferation in interleukin‐2, diverse T‐cell‐receptor‐Vβ repertoire and polarized interferon‐γ expression

V Badovinac, C Boggiano, V Trajković, A B Frey, N L Vujanović, D P Gold, M Mostarica-Stojković and S Vukmanović

Immunology, Vol.95(1), pp.117-125

09/1998

DOI: 10.1046/j.1365-2567.1998.00567.x

PMID: 9767466

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https://www.ncbi.nlm.nih.gov/pmc/articles/1364385View

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Abstract

Cells expressing markers of both natural killer and T lymphocytes (NK T cells) in humans and mice express a restricted T-cell receptor (TCR) repertoire, are of CD4- CD8- or CD4+ CD8- phenotype, and upon anti-CD3 stimulation secrete large amounts of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma). NK T cells may be the primary source of IL-4-promoting T helper type 2 (Th2) responses and/or they might be involved in regulating the balance between Th1- and Th2-type immune responses, and may consequently affect susceptibility to autoimmune diseases associated with a skewed Th phenotype. We show that rat NK T cells selectively proliferate to IL-2, and use this fact to analyse cytokine production by NK T cells in two rat strains differentially susceptible to Th1- or Th2-type autoimmune diseases. Analysis by reverse transcription-polymerase chain reaction revealed that, in contrast to mouse, rat NK T cells secrete exclusively IFN-gamma and not IL-4 after anti-CD3 stimulation, and use a wider TCR-Vbeta repertoire, suggesting that rat NK T cells are not essential for the development of Th2-type CD4+ T-cell responses.

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Title: Subtitle: Rat NKR‐P1+CD3+T cells: selective proliferation in interleukin‐2, diverse T‐cell‐receptor‐Vβ repertoire and polarized interferon‐γ expression
Creators: V Badovinac - Institute for Biological Research, Belgrade, Yugoslavia
C Boggiano - Sidney Kimmel Cancer Institute, Science Park Road, San Diego, CA, USA
V Trajković - Institute for Biological Research, Belgrade, Yugoslavia
A B Frey - Department of Cell Biology and Kaplan Comprehensive Cancer Center, NYU Medical Center, New York, NY USA
N L Vujanović - Department of Pathology, Pittsburgh Cancer Institute, DeSoto and O’Hara, BST, W940, Pittsburgh, PA, USA
D P Gold - Sidney Kimmel Cancer Institute, Science Park Road, San Diego, CA, USA
M Mostarica-Stojković - Institute of Microbiology and Immunology, Belgrade University School of Medicine, Belgrade, Yugoslavia
S Vukmanović - Michael Heidelberger Division of Immunology, Department of Pathology, and Kaplan Comprehensive Cancer Center, NYU Medical Center, New York, NY, USA
Resource Type: Journal article
Publication Details: Immunology, Vol.95(1), pp.117-125
DOI: 10.1046/j.1365-2567.1998.00567.x
PMID: 9767466
NLM abbreviation: Immunology
ISSN: 0019-2805
eISSN: 1365-2567
Language: English
Date published: 09/1998
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984046919902771

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