Journal article
Reactions of 4-hydroxynonenal with proteins and cellular targets
Free radical biology & medicine, Vol.37(7), pp.937-945
2004
DOI: 10.1016/j.freeradbiomed.2004.06.012
PMID: 15336309
Abstract
Peroxidative degradation of lipids yields the aldehyde 4-hydroxy-2-nonenal (4HNE) as a major product. The lipid aldehyde is an electrophile, and reactivity of 4HNE toward protein nucleophiles (i.e., Cys, His, and Lys) has been characterized. Through the use of purified enzymes and isolated cells, various pathways for biotransformation of the lipid aldehyde have been identified and include enzyme-mediated oxidation, reduction, and glutathione conjugation. Uncontrolled oxidative stress can yield excessive lipid peroxidation and 4HNE generation, however, and overwhelm these cellular defenses. Indeed, in vitro and in vivo production of 4HNE in response to pro-oxidant exposure has been demonstrated using antibodies to protein adducts of the lipid aldehyde. Recent evidence suggests a role for protein modification by 4HNE in the pathogenesis of several diseases (e.g., alcohol-induced liver disease); however, the precise mechanism(s) is currently unknown but likely results from adduction of proteins involved in cellular homeostasis or biological signaling.
Details
- Title: Subtitle
- Reactions of 4-hydroxynonenal with proteins and cellular targets
- Creators
- Dennis R PetersenJonathan A Doorn
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.37(7), pp.937-945
- DOI
- 10.1016/j.freeradbiomed.2004.06.012
- PMID
- 15336309
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2004
- Academic Unit
- Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984070955802771
Metrics
14 Record Views