Logo image
Reactivity of b72.3 with adenocarcinomas. An immunohistochemical study of 476 cases
Journal article   Peer reviewed

Reactivity of b72.3 with adenocarcinomas. An immunohistochemical study of 476 cases

Timothy S Loy and Marcus B Nashelsky
Cancer, Vol.72(8), pp.2495-2498
10/15/1993
DOI: 10.1002/1097-0142(19931015)72:8<2495::AID-CNCR2820720830>3.0.CO;2-E
PMID: 8402466

View Online

Abstract

Background. The monoclonal antibody B72.3 has been shown to react with several common types of adenocarcinoma. However, the distribution of B72.3 reactivity in adenocarcinomas has not been well‐enough defined to determine whether B72.3 immunostaining would be helpful in the diagnosis of adenocarcinoma of unknown origin. Methods. Formaldehyde fixed, paraffin‐embedded tissue from 476 adenocarcinomas from a variety of primary sites were studied using B72.3 and an avidin‐biotin complex immunohistochemical technique. Results. B72.3 immunoreactivity was seen in 75–100% of the adenocarcinomas from the ovary, endocervix, endometrium, distal esophagus/stomach, colon, lung, breast, prostate, pancreas, and bile ducts. Positive reactions were unusual in thyroid carcinomas (7%) and renal cell carcinomas (11%). B72.3 reactivity was not seen in the 20 hepatocellular carcinomas or 4 adrenal cortical carcinomas studied. Conclusions. B72.3 reacts with a wide variety of adenocarcinomas. Immunostaining with B72.3 may be helpful in excluding hepatocellular carcinoma in certain clinical settings.
tumor‐associated glycoprotein B72.3 adenocarcinoma immunocytochemistry immunohistochemistry monoclonal antibody hepatocellular carcinoma

Details

Logo image