Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor

Zhuang Hao; Yibo Zhu; Xuejun Wang; Pavana G Rotti; Christopher DiMarco; Scott R Tyler; Xuezeng Zhao; John F Engelhardt; James Hone; Qiao Lin

doi:10.1021/acsami.7b07684

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Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor

Journal article

Peer reviewed

Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor

Zhuang Hao, Yibo Zhu, Xuejun Wang, Pavana G Rotti, Christopher DiMarco, Scott R Tyler, Xuezeng Zhao, John F Engelhardt, James Hone and Qiao Lin

ACS applied materials & interfaces, Vol.9(33), pp.27504-27511

08/23/2017

DOI: 10.1021/acsami.7b07684

PMCID: PMC7875320

PMID: 28770993

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https://www.ncbi.nlm.nih.gov/pmc/articles/7875320View

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Abstract

This paper presents an approach to the real-time, label-free, specific, and sensitive monitoring of insulin using a graphene aptameric nanosensor. The nanosensor is configured as a field-effect transistor, whose graphene-based conducting channel is functionalized with a guanine-rich IGA3 aptamer. The negatively charged aptamer folds into a compact and stable antiparallel or parallel G-quadruplex conformation upon binding with insulin, resulting in a change in the carrier density, and hence the electrical conductance, of the graphene. The change in the electrical conductance is then measured to enable the real-time monitoring of insulin levels. Testing has shown that the nanosensor offers an estimated limit of detection down to 35 pM and is functional in Krebs-Ringer bicarbonate buffer, a standard pancreatic islet perfusion medium. These results demonstrate the potential utility of this approach in label-free monitoring of insulin and in timely prediction of accurate insulin dosage in clinical diagnostics.

Biosensing Techniques

G-Quadruplexes

Graphite

Insulin - chemistry

Islets of Langerhans

Details

Title: Subtitle: Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor
Creators: Zhuang Hao - Columbia University
Yibo Zhu - Columbia University
Xuejun Wang - Columbia University
Pavana G Rotti
Christopher DiMarco - Columbia University
Scott R Tyler
Xuezeng Zhao - Harbin Institute of Technology
John F Engelhardt
James Hone - Columbia University
Qiao Lin - Columbia University
Resource Type: Journal article
Publication Details: ACS applied materials & interfaces, Vol.9(33), pp.27504-27511
DOI: 10.1021/acsami.7b07684
PMID: 28770993
PMCID: PMC7875320
NLM abbreviation: ACS Appl Mater Interfaces
ISSN: 1944-8244
eISSN: 1944-8252
Grant note: R33 CA196470 / NCI NIH HHS DP3 DK101085 / NIDDK NIH HHS
Language: English
Date published: 08/23/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984284342602771

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