Journal article
Real-Time PCR Quantification of Mycobacterium immunogenum in Used Metalworking Fluids
Journal of occupational and environmental hygiene, Vol.5(12), pp.755-760
11/04/2008
DOI: 10.1080/15459620802446343
PMID: 18821262
Abstract
Rapid detection and quantification of Mycobacterium immunogenum in field samples of metalworking fluids (MWFs) is important for factory fluid surveillance programs. The applicability of the developed DNA extraction and quantitative real-time PCR (qPCR) methods to detect and quantify M. immunogenum in used MWFs was evaluated. Total DNA from these samples was extracted, and M. immunogenum measured by qPCR by comparison with a standard curve derived from plasmid vectors. PCR counts were compared with bacterial culture counts. PCR counts of M. immunogenum varied from 1.42 × 10
3
to 3.68 × 10
6
cells/mL of MWFs. Recovery of M. immunogenum by bacterial culture varied from 2.5% to 70% of qPCR count in corresponding samples. Quantitative PCR could be used to measure M. immunogenum load in MWF samples with greater sensitivity and shorter processing time than the classic bacterial culture-based approach. The proposed qPCR approach could be routinely used in real-time PCR-equipped laboratories to provide early detection of M. immunogenum and to control proliferation that probably leads to hypersensitivity pneumonitis in exposed workers.
Details
- Title: Subtitle
- Real-Time PCR Quantification of Mycobacterium immunogenum in Used Metalworking Fluids
- Creators
- M Veillette - Institut Universitaire de cardiologie et de pneumologie de l'Hôpital Laval, Université LavalG Pagé - Institut Universitaire de cardiologie et de pneumologie de l'Hôpital Laval, Université LavalP. S Thorne - University of Iowa, College of Public HealthC Duchaine - Université Laval, Département de biochimie et microbiologie
- Resource Type
- Journal article
- Publication Details
- Journal of occupational and environmental hygiene, Vol.5(12), pp.755-760
- Publisher
- Taylor & Francis Group
- DOI
- 10.1080/15459620802446343
- PMID
- 18821262
- ISSN
- 1545-9624
- eISSN
- 1545-9632
- Language
- English
- Date published
- 11/04/2008
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983997326102771
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