Journal article
Real-Time Visualization of Mycobacterium-Macrophage Interactions Leading to Initiation of Granuloma Formation in Zebrafish Embryos
Immunity (Cambridge, Mass.), Vol.17(6), pp.693-702
2002
DOI: 10.1016/S1074-7613(02)00475-2
PMID: 12479816
Abstract
Infection of vertebrate hosts with pathogenic Mycobacteria, the agents of tuberculosis, produces granulomas, highly organized structures containing differentiated macrophages and lymphocytes, that sequester the pathogen. Adult zebrafish are naturally susceptible to tuberculosis caused by
Mycobacterium marinum. Here, we exploit the optical transparency of zebrafish embryos to image the events of
M. marinum infection in vivo. Despite the fact that the embryos do not yet have lymphocytes, infection leads to the formation of macrophage aggregates with pathological hallmarks of granulomas and activation of previously identified granuloma-specific
Mycobacterium genes. Thus,
Mycobacterium-macrophage interactions can initiate granuloma formation solely in the context of innate immunity. Strikingly, infection can redirect normal embryonic macrophage migration, even recruiting macrophages seemingly committed to their developmentally dictated tissue sites.
Details
- Title: Subtitle
- Real-Time Visualization of Mycobacterium-Macrophage Interactions Leading to Initiation of Granuloma Formation in Zebrafish Embryos
- Creators
- J.Muse Davis - Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195 USAHilary Clay - Molecular and Cellular Biology Graduate Program, University of Washington School of Medicine, Seattle, WA 98195 USAJessica L Lewis - Molecular and Cellular Biology Graduate Program, University of Washington School of Medicine, Seattle, WA 98195 USANafisa Ghori - Electron Microscopy Center, Stanford University School of Medicine, Stanford, CA 94305 USAPhilippe Herbomel - Unité Macrophages et Développement de l'Immunité, Institut Pasteur, 75015 Paris, FranceLalita Ramakrishnan - Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195 USA
- Resource Type
- Journal article
- Publication Details
- Immunity (Cambridge, Mass.), Vol.17(6), pp.693-702
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S1074-7613(02)00475-2
- PMID
- 12479816
- ISSN
- 1074-7613
- eISSN
- 1097-4180
- Language
- English
- Date published
- 2002
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093322702771
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