Journal article
Real-time transposable element activity in individual live cells
Proceedings of the National Academy of Sciences - PNAS, Vol.113(26), pp.7278-7283
06/28/2016
DOI: 10.1073/pnas.1601833113
PMCID: PMC4932956
PMID: 27298350
Abstract
The excision and reintegration of transposable elements (TEs) restructure their host genomes, generating cellular diversity involved in evolution, development, and the etiology of human diseases. Our current knowledge of TE behavior primarily results from bulk techniques that generate time and cell ensemble averages, but cannot capture cell-to-cell variation or local environmental and temporal variability. We have developed an experimental system based on the bacterial TE IS608 that uses fluorescent reporters to directly observe single TE excision events in individual cells in real time. We find that TE activity depends upon the TE's orientation in the genome and the amount of transposase protein in the cell. We also find that TE activity is highly variable throughout the lifetime of the cell. Upon entering stationary phase, TE activity increases in cells hereditarily predisposed to TE activity. These direct observations demonstrate that real-time live-cell imaging of evolution at the molecular and individual event level is a powerful tool for the exploration of genome plasticity in stressed cells.
Details
- Title: Subtitle
- Real-time transposable element activity in individual live cells
- Creators
- Neil H Kim - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801Gloria Lee - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801Nicholas A Sherer - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801K Michael Martini - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801Nigel Goldenfeld - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Institute for Universal Biology NASA Astrobiology Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801; tkuhlman@illinois.edu nigel@uiuc.eduThomas E Kuhlman - Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, IL 61801; Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 tkuhlman@illinois.edu nigel@uiuc.edu
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.113(26), pp.7278-7283
- DOI
- 10.1073/pnas.1601833113
- PMID
- 27298350
- PMCID
- PMC4932956
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences; United States
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation, award: PHY 1430124; DOI: 10.13039/100000879, name: Alfred P. Sloan Foundation, award: FG-2015-65532; DOI: 10.13039/100012627, name: NASA Astrobiology Institute, award: NNA13AA91A; DOI: 10.13039/100000001, name: National Science Foundation, award: DGE-1144245
- Language
- English
- Date published
- 06/28/2016
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984065481702771
Metrics
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