Journal article
Reappraisal or regional thiamine content in the central nervous system of the normal and thiamine-deficient mice
Metabolic brain disease, Vol.8(1), pp.45-59
1993
DOI: 10.1007/BF01000529
PMID: 8492784
Abstract
The regional distribution of thiamine and its phosphate esters was measured in the central nervous system (CNS) of normal and thiamine-deficient mice. Twelve small areas were punched out from frozen sections and they were individually analyzed by high performance liquid chromatography (HPLC). Regional difference was noted in both the content and ratio of thiamine and its phosphate esters in the normal CNS. In pyrithiamine-induced thiamine deficiency, thiamine pyrophosphate (TPP) content in all the areas was reduced to less than 13% of the control values on day 10, when the neurological signs developed. Although there were considerable regional variations in the reduction rate of thiamine and its phosphate esters, no correlation was established between the severity of tissue damage and the magnitude of thiamine reduction in individual areas. These results indicate that a derangement in TPP-dependent processes, either alone or in combination with other factors, plays a more critical role in the neuronal damage under thiamine deficiency than depletion of thiamine compounds per se.
Details
- Title: Subtitle
- Reappraisal or regional thiamine content in the central nervous system of the normal and thiamine-deficient mice
- Creators
- N HARATA - Tohoku univ. school medicine, dep. neurological sci., Aoba-ku Sendai 980, JapanY IWASAKI - Tohoku univ. school medicine, dep. neurological sci., Aoba-ku Sendai 980, JapanY OHARA - Tohoku univ. school medicine, dep. neurological sci., Aoba-ku Sendai 980, Japan
- Resource Type
- Journal article
- Publication Details
- Metabolic brain disease, Vol.8(1), pp.45-59
- Publisher
- Springer; New York, NY
- DOI
- 10.1007/BF01000529
- PMID
- 8492784
- ISSN
- 0885-7490
- eISSN
- 1573-7365
- Language
- English
- Date published
- 1993
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025669502771
Metrics
11 Record Views