Recent advances in 3D printing with protein-based inks

Xuan Mu; Francesca Agostinacchio; Ning Xiang; Ying Pei; Yousef Khan; Chengchen Guo; Peggy Cebe; Antonella Motta; David L Kaplan

doi:10.1016/j.progpolymsci.2021.101375

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Recent advances in 3D printing with protein-based inks

Journal article

Open access

Peer reviewed

Recent advances in 3D printing with protein-based inks

Xuan Mu, Francesca Agostinacchio, Ning Xiang, Ying Pei, Yousef Khan, Chengchen Guo, Peggy Cebe, Antonella Motta and David L Kaplan

Progress in polymer science, Vol.115, p.101375

04/2021

DOI: 10.1016/j.progpolymsci.2021.101375

PMCID: PMC7996313

PMID: 33776158

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Abstract

Three-dimensional (3D) printing is a transformative manufacturing strategy, allowing rapid prototyping, customization, and flexible manipulation of structure-property relationships. Proteins are particularly appealing to formulate inks for 3D printing as they serve as essential structural components of living systems, provide a support presence in and around cells and for tissue functions, and also provide the basis for many essential ex vivo secreted structures in nature. Protein-based inks are beneficial in vivo due to their mechanics, chemical and physical match to the specific tissue, and full degradability, while also to promoting implant-host integration and serving as an interface between technology and biology. Exploiting the biological, chemical, and physical features of protein-based inks can provide key opportunities to meet the needs of tissue engineering and regenerative medicine. Despite these benefits, protein-based inks impose nontrivial challenges to 3D printing, such as sufficient concentration, rheological features, and structural hierarchy observed in nature that is a source of the robust mechanics and functions of these materials. This review introduces photo-crosslinking mechanisms and rheological principles that underpins a variety of 3D printing techniques. The review also highlights recent advances in the design, development, and biomedical utility of monolithic and composite inks from a range of proteins, including collagen, silk, fibrinogen, and others. One particular focus throughout the review is to introduce unique material characteristics of proteins, including amino acid sequences, molecular assembly, and secondary conformations for designing printing inks and for controlling the printed structures. Future perspectives of 3D printing with protein-based inks are also provided to support the promising spectrum of biomedical research accessible to these materials. [Display omitted]

Collagen

Directed assembly

Fibrinogen

Photopolymerization

Rheology

Silk

Simulation

Details

Title: Subtitle: Recent advances in 3D printing with protein-based inks
Creators: Xuan Mu - Tufts University
Francesca Agostinacchio - University of Trento
Ning Xiang - Tufts University
Ying Pei - Zhengzhou University
Yousef Khan - Tufts University
Chengchen Guo - Tufts University
Peggy Cebe - Tufts University
Antonella Motta - University of Trento
David L Kaplan - Tufts University
Resource Type: Journal article
Publication Details: Progress in polymer science, Vol.115, p.101375
DOI: 10.1016/j.progpolymsci.2021.101375
PMID: 33776158
PMCID: PMC7996313
NLM abbreviation: Prog Polym Sci
ISSN: 0079-6700
eISSN: 1873-1619
Publisher: Elsevier B.V
Grant note: DOI: 10.13039/100000001, name: National Science Foundation, award: DMR-1608125; DOI: 10.13039/100000002, name: National Institutes of Health, award: P41EB002520, R01EB021264, R01NS092847, U01EB014976; DOI: 10.13039/100000181, name: Air Force Office of Scientific Research, award: FA9550-17-1-0333
Language: English
Date published: 04/2021
Academic Unit: Roy J. Carver Department of Biomedical Engineering
Record Identifier: 9984276451102771

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