Journal article
Receptor‐Linked Hydrolysis of Phosphoinositides and Production of Prostacyclin in Cerebral Endothelial Cells
Journal of neurochemistry, Vol.58(5), pp.1930-1935
05/1992
DOI: 10.1111/j.1471-4159.1992.tb10071.x
PMID: 1313855
Abstract
: The receptor agonist‐mediated hydrolysis of phosphoinositides and production of prostacyclin were studied in murine cerebral endothelial cells (MCEC). Of 11 neurotransmitters and neuromodulators examined, carbachol, noradrenaline (NE), bradykinin, and thrombin significantly increased 3H‐inositol phosphate accumulation in the presence of LiCl (20 mM). The maximal stimulation of [3H]inositol monophosphate ([3H]IP1) reached approximately 11, 11, seven, and four times the basal levels for carbachol, NE, bradykinin, and thrombin, respectively. The EC50 values of IP1 accumulation for carbachol and NE were 34 and 0.16 μM, respectively. The muscarinic antagonists, atropine and pirenzepine, blocked the carbachol‐induced IP1 accumulation with Ki values of 0.3 and 30 nM, respectively. The adrenergic antagonist, prazosin, blocked NE‐induced IP1 accumulation with a Ki of 0.1 nM. The calcium ionophore A23187, histamine, glutamate, vasopressin, serotonin, platelet activating factor, and substance P did not stimulate IP1 accumulation. A23187, bradykinin, and thrombin stimulated prostacyclin release to approximately four, four, and two times the basal levels, respectively, whereas carbachol and NE had little effect upon prostacyclin release. These results suggest that the activation of phospholipase C and of phospholipase A2 in MCEC are regulated separately.
Details
- Title: Subtitle
- Receptor‐Linked Hydrolysis of Phosphoinositides and Production of Prostacyclin in Cerebral Endothelial Cells
- Creators
- Jian XuZhi‐Xiang QuSteven A MooreChung Y HsuEdward L Hogan
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.58(5), pp.1930-1935
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- DOI
- 10.1111/j.1471-4159.1992.tb10071.x
- PMID
- 1313855
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Number of pages
- 6
- Language
- English
- Date published
- 05/1992
- Academic Unit
- Pathology
- Record Identifier
- 9984047978702771
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