Journal article
Reciprocal Efficiency of RNQ1 and Polyglutamine Detoxification in the Cytosol and Nucleus
Molecular biology of the cell, Vol.20(19), pp.4162-4173
10/2009
DOI: 10.1091/mbc.e09-02-0170
PMCID: PMC2754930
PMID: 19656852
Abstract
Onset of proteotoxicity is linked to change in the subcellular location of proteins that cause misfolding diseases. Yet, factors that drive changes in disease protein localization and the impact of residence in new surroundings on proteotoxicity are not entirely clear. To address these issues, we examined aspects of proteotoxicity caused by Rnq1-green fluorescent protein (GFP) and a huntingtin's protein exon-1 fragment with an expanded polyglutamine tract (Htt-103Q), which is dependent upon the intracellular presence of [RNQ+] prions. Increasing heat-shock protein 40 chaperone activity before Rnq1-GFP expression, shifted Rnq1-GFP aggregation from the cytosol to the nucleus. Assembly of Rnq1-GFP into benign amyloid-like aggregates was more efficient in the nucleus than cytosol and nuclear accumulation of Rnq1-GFP correlated with reduced toxicity. [RNQ+] prions were found to form stable complexes with Htt-103Q, and nuclear Rnq1-GFP aggregates were capable of sequestering Htt-103Q in the nucleus. On accumulation in the nucleus, conversion of Htt-103Q into SDS-resistant aggregates was dramatically reduced and Htt-103Q toxicity was exacerbated. Alterations in activity of molecular chaperones, the localization of intracellular interaction partners, or both can impact the cellular location of disease proteins. This, in turn, impacts proteotoxicity because the assembly of proteins to a benign state occurs with different efficiencies in the cytosol and nucleus.
Details
- Title: Subtitle
- Reciprocal Efficiency of RNQ1 and Polyglutamine Detoxification in the Cytosol and Nucleus
- Creators
- Peter M Douglas - Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7090Daniel W Summers - Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7090Hong-Yu Ren - Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7090Douglas M Cyr - Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7090
- Contributors
- Ramanujan S Hegde (Editor)
- Resource Type
- Journal article
- Publication Details
- Molecular biology of the cell, Vol.20(19), pp.4162-4173
- DOI
- 10.1091/mbc.e09-02-0170
- PMID
- 19656852
- PMCID
- PMC2754930
- NLM abbreviation
- Mol Biol Cell
- ISSN
- 1059-1524
- eISSN
- 1939-4586
- Publisher
- American Society for Cell Biology
- Language
- English
- Date published
- 10/2009
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070768302771
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