Reciprocal age related change in natural killer cell receptors for MHC class I

Charles T Lutz; Mikel B Moore; Sarah Bradley; Brent J Shelton; Susan K Lutgendorf

doi:10.1016/j.mad.2005.01.004

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Reciprocal age related change in natural killer cell receptors for MHC class I

Journal article

Peer reviewed

Reciprocal age related change in natural killer cell receptors for MHC class I

Charles T Lutz, Mikel B Moore, Sarah Bradley, Brent J Shelton and Susan K Lutgendorf

Mechanisms of ageing and development, Vol.126(6), pp.722-731

2005

DOI: 10.1016/j.mad.2005.01.004

PMCID: PMC3394430

PMID: 15888327

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Abstract

Natural killer (NK) cells are essential for healthy aging. NK cell activation is controlled by MHC class I-specific CD94/NKG2 receptors and killer immunoglobulin-like receptors (KIR). To assess NK cytotoxic function in isolation from MHC receptor engagement, we measured the ability of purified NK cells to kill mouse P815 target cells in the presence of anti-CD16 mAb. CD16-mediated cytotoxicity did not change with age, indicating that NK activation and cytotoxic granule release remained functional. We then investigated MHC class I receptor expression on NK cells. There was an age related decrease in CD94 and NKG2A expression and a reciprocal age related increase in KIR expression. NKG2A expression also declined with age on CD56 + T cells. CD94/NKG2A receptor function was proportional to expression, indicating that NK cell inhibitory signaling pathways were intact. NKG2A and KIR expression were complementary, suggesting that CD94/NKG2A function could substitute for inhibitory KIR function during polyclonal NK cell development in both young and elderly adults. The distinct roles of CD94/NKG2A and KIR receptors suggest that shifting MHC class I receptor expression patterns reflect age related changes in NK cell and CD56 + T cell turnover and function in vivo.

CD94

Natural killer

Receptor

Kiribati

NKT

NKG2A

Aging

MHC class I

Cytotoxicity

Details

Title: Subtitle: Reciprocal age related change in natural killer cell receptors for MHC class I
Creators: Charles T Lutz - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA
Mikel B Moore - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA
Sarah Bradley - Department of Psychology, University of Iowa, Iowa City, IA 52242, USA
Brent J Shelton - Markey Cancer Center, University of Kentucky College of Medicine, Lexington, KY 40536, USA
Susan K Lutgendorf - Department of Psychology, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Mechanisms of ageing and development, Vol.126(6), pp.722-731
DOI: 10.1016/j.mad.2005.01.004
PMID: 15888327
PMCID: PMC3394430
NLM abbreviation: Mech Ageing Dev
ISSN: 0047-6374
eISSN: 1872-6216
Publisher: Elsevier Ireland Ltd
Language: English
Date published: 2005
Academic Unit: Psychological and Brain Sciences; Iowa Neuroscience Institute; Obstetrics and Gynecology; Urology
Record Identifier: 9984065769702771

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