Journal article
Recombinant Interferons β and γ Have a Higher Antiviral Activity than Interferon-α in Coxsackievirus B3-Infected Carrier State Cultures of Human Myocardial Fibroblasts
Journal of interferon & cytokine research, Vol.16(4), pp.283-287
04/1996
DOI: 10.1089/jir.1996.16.283
PMID: 9162521
Abstract
We compared the antiviral activities of three recombinant human interferons (IFN-alph2a, IFN-beta, and IFN-gamma) in cultured human myocardial fibroblasts to select a candidate for trial in heart disease induced by cardiotropic enterovirus, e.g., coxsackievirus B3 (CVB3). Cells were exposed to CVB3, and after 7 days, when a persistent infection had developed, IFN was added. Virus yields were measured on alternate days for the next 7 or 16 days, and IFN activity was assessed as the percentage reduction in yield. IFN-gamma and IFN-beta were both highly active and reduced virus yields by 2 log (EC(99)) at concentrations of 23.4 IU/ml (SD = 8.6) and 10.1 IU/ml (SD = 3.2), respectively; with 250 IU/ml of either IFN, no infectious virus was formed. Unexpectedly, IFN-alpha2a (EC(99)> 1250 IU/ml) was at least 120 times less active than IFN-beta; after use for 8 days or more, the minor effects it produced were no longer related to the concentration applied. Despite the pharmacokinetic advantages of IFN-alpha2a, our data suggest that IFN-beta should in preference be evaluated in the clinic.
Details
- Title: Subtitle
- Recombinant Interferons β and γ Have a Higher Antiviral Activity than Interferon-α in Coxsackievirus B3-Infected Carrier State Cultures of Human Myocardial Fibroblasts
- Creators
- ALBERT HEIMMICHAEL STILLE-SIEGENERPATRICIA PRING-ÅKERBLOMISABELLA GRUMBACHCLAUDIA BREHMHEINRICH KREUZERHANS-REINER FIGULLA
- Resource Type
- Journal article
- Publication Details
- Journal of interferon & cytokine research, Vol.16(4), pp.283-287
- DOI
- 10.1089/jir.1996.16.283
- PMID
- 9162521
- ISSN
- 1079-9907
- eISSN
- 1557-7465
- Language
- English
- Date published
- 04/1996
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984094577502771
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