Journal article
Recombinant Leishmania Hsp90 and Hsp70 are recognized by sera from visceral leishmaniasis patients but not Chagas' disease patients
Journal of clinical microbiology, Vol.30(2), pp.330-335
02/1992
DOI: 10.1128/jcm.30.2.330-335.1992
PMCID: PMC265055
PMID: 1537901
Abstract
Approximately 70% of the cDNA clones identified by immunoscreening Leishmania donovani expression libraries with serum from a patient with visceral leishmaniasis (kala-azar) were found to encode the highly conserved Hsp90 and Hsp70 members of the heat shock protein family. Recombinant fusion proteins containing the C-terminal portions of L. donovani Hsp90 and Hsp70 were used as target antigens in enzyme-linked immunosorbent assays of various sera. Sera from four patients with visceral leishmaniasis recognized recombinant Leishmania Hsp90 and Hsp70, while sera from seven patients with Chagas' disease did not, despite the fact that Trypanosoma cruzi Hsp90 and Hsp70 share more than 80% amino acid identity with their counterparts in Leishmania spp. Thus, Leishmania Hsp90 and Hsp70 elicit strong humoral responses and are potential candidates for specific serodiagnostic assays capable of distinguishing between L. donovani and T. cruzi infections.
Details
- Title: Subtitle
- Recombinant Leishmania Hsp90 and Hsp70 are recognized by sera from visceral leishmaniasis patients but not Chagas' disease patients
- Creators
- C R de Andrade - University of IowaL V Kirchhoff - University of IowaJ E Donelson - University of IowaK Otsu - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.30(2), pp.330-335
- DOI
- 10.1128/jcm.30.2.330-335.1992
- PMID
- 1537901
- PMCID
- PMC265055
- NLM abbreviation
- J Clin Microbiol
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Language
- English
- Date published
- 02/1992
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280837702771
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