Journal article
Recombinant hepatitis C virus-like particles expressed by baculovirus: Utility in cell-binding and antibody detection assays
Journal of medical virology, Vol.68(4), pp.537-543
12/2002
DOI: 10.1002/jmv.10237
PMID: 12376962
Abstract
Hepatitis C virus (HCV) is difficult to study due to the lack of an efficient cell culture system or small animal model. As a result, HCV–cell interactions are not well-defined. In addition, several studies have identified a subset of patients in whom HCV RNA is present, but HCV antibody is not detected. We produced recombinant baculoviruses that expressed HCV structural proteins (core, E1 and E2, nt 342–2651) or control proteins. The HCV structural protein precursor was processed into immunoreactive proteins of appropriate size, and sucrose density sedimentation and electron microscopy of infected cell lysates demonstrated particle formation. To evaluate HCV antigenicity, particularly in patients who tested negative for HCV antibody in commercial HCV immunoassays but had persistent viremia, we evaluated the virus-like particles (VLPs) in solid-phase immunoassays. VLPs reacted with sera from HCV antibody positive subjects in these solid phase immunoassays, but not with control sera. Plasma samples from 19% (5/26) of HCV antibody negative subjects who were persistently HCV RNA positive also reacted with the HCV VLPs. When incubated with MOLT-4 cells at 4°C, HCV VLPs demonstrated cell binding, and behaved similar to plasma-derived HCV preparations in a flow cytometry-based cell binding assay. These data suggest that recombinant HCV VLPs may allow identification of HCV antibody in patients, including some patients with persistent viremia and who are seronegative with current assays. In addition, HCV VLPs seem useful for evaluating HCV–cell interactions.
Details
- Title: Subtitle
- Recombinant hepatitis C virus-like particles expressed by baculovirus: Utility in cell-binding and antibody detection assays
- Creators
- Jinhua Xiang - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaSabina Wünschmann - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaSarah L George - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaDonna Klinzman - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaWarren N Schmidt - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaDouglas R LaBrecque - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, IowaJack T Stapleton - Departments of Internal Medicine and Research, Iowa City Veterans Administration Medical Center and The University of Iowa College of Medicine, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of medical virology, Vol.68(4), pp.537-543
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jmv.10237
- PMID
- 12376962
- ISSN
- 0146-6615
- eISSN
- 1096-9071
- Number of pages
- 7
- Grant note
- name: Veterans Administration; name: NIH, award: AA12671, AI50478, AA 13215, RR00059; name: University of Iowa, Central Investment Fund for Research and Education (CIFRE), award: 5-0251123
- Language
- English
- Date published
- 12/2002
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094329602771
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