Journal article
Recombinant protein synthesis in Trichoplusia ni BTI‐Tn‐5B1‐4 insect cell aggregates
Biotechnology and bioengineering, Vol.63(5), pp.612-617
06/05/1999
DOI: 10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
Abstract
The Trichoplusia ni BTI‐Tn‐5B1‐4 (Tn‐5B1‐4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn‐5B1‐4 cell aggregates and cells selected to grow predominantly as individual cells. No significant difference was found between cell aggregates and cells growing predominantly individually in regard to cell growth rate, glucose consumption and lactate accumulation, and specific recombinant protein synthesis levels. In addition, the levels of recombinant protein synthesis were considerably higher than those produced by the commonly used Spodoptera frugiperda Sf‐9 insect cell line. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 612–617, 1999.
Details
- Title: Subtitle
- Recombinant protein synthesis in Trichoplusia ni BTI‐Tn‐5B1‐4 insect cell aggregates
- Creators
- Mark A SaarinenKimberly A TroutnerSteve G GladdenChristine M Mitchell‐LogeanDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.63(5), pp.612-617
- Publisher
- John Wiley & Sons, Inc; New York
- DOI
- 10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Number of pages
- 6
- Grant note
- NASA (NAG9‐824) NSF (BES‐9318670)
- Language
- English
- Date published
- 06/05/1999
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003476602771
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