Journal article
Reconstitution of tracheal grafts with a genetically modified epithelium
Proceedings of the National Academy of Sciences - PNAS, Vol.88(24), pp.11192-11196
12/15/1991
DOI: 10.1073/pnas.88.24.11192
PMCID: PMC53100
PMID: 1763032
Abstract
A rational approach to the development of gene therapies for cystic fibrosis requires a better understanding of the cellular targets for gene transfer in the airway epithelium. We have used recombinant retroviruses to study the dynamics and lineage relationships of a regenerating rat tracheal epithelium. Primary cultures of tracheal epithelial cells were exposed to lacZ-transducing retroviruses and subsequently seeded into denuded trachea that were implanted into BALB/c (nu/nu) mice. The grafts developed a fully differentiated mucociliary epithelium containing large clones of lacZ-expressing cells with virtually all cell types represented within each clone. These data are most consistent with gene transfer into a putative progenitor cell that is capable of extensive self renewal and pleuripotent development. Vector-specific variation in transgene expression was noted in the various cell types.
Details
- Title: Subtitle
- Reconstitution of tracheal grafts with a genetically modified epithelium
- Creators
- J F Engelhardt - University of MichiganE D Allen - University of MichiganJ M Wilson - University of Michigan
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.88(24), pp.11192-11196
- DOI
- 10.1073/pnas.88.24.11192
- PMID
- 1763032
- PMCID
- PMC53100
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 12/15/1991
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984284336602771
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