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Recruitment of HIV and its receptors to dendritic cell-T cell junctions
Journal article   Peer reviewed

Recruitment of HIV and its receptors to dendritic cell-T cell junctions

David McDonald, Li Wu, Stacy M Bohks, Vineet N KewalRamani, Derya Unutmaz and Thomas J Hope
Science (American Association for the Advancement of Science), Vol.300(5623), pp.1295-1297
05/23/2003
DOI: 10.1126/science.1084238
PMID: 12730499

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Abstract

Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.
Green Fluorescent Proteins Coculture Techniques Humans Dendritic Cells - physiology CD4-Positive T-Lymphocytes - ultrastructure CD4-Positive T-Lymphocytes - physiology Luminescent Proteins - analysis Receptors, CCR5 - metabolism HIV-1 - physiology CD4-Positive T-Lymphocytes - virology Dendritic Cells - virology Luciferases - analysis vpr Gene Products, Human Immunodeficiency Virus Virion - physiology Cells, Cultured Cell Adhesion Gene Products, vpr - analysis Receptors, CXCR4 - metabolism Monocytes Receptors, HIV - metabolism Dendritic Cells - ultrastructure Models, Biological Fluorescent Antibody Technique Lipopolysaccharides - pharmacology Intercellular Junctions - virology Cell Membrane - virology Intercellular Junctions - physiology CD4 Antigens - metabolism

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