Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization

Kailey Harrell; Madison Day; Sarit Smolikove

doi:10.1016/j.mrfmmm.2021.111743

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Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization

Journal article

Peer reviewed

Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization

Kailey Harrell, Madison Day and Sarit Smolikove

Mutation research, Vol.822, pp.111743-111743

04/20/2021

DOI: 10.1016/j.mrfmmm.2021.111743

PMCID: PMC8176624

PMID: 33975127

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Abstract

DNA double-strand breaks (DSBs) are one of the most dangerous assaults on the genome, and yet their natural and programmed production are inherent to life. When DSBs arise close together they are particularly deleterious, and their repair may require an altered form of the DNA damage response. Our understanding of how clustered DSBs are repaired in the germline is unknown. Using laser microirradiation, we examine early events in the repair of clustered DSBs in germ cells within Caenorhabditis elegans. We use precise temporal resolution to show how the recruitment of MRE-11 to complex damage is regulated, and that clustered DNA damage can recruit proteins from various repair pathways. Abrogation of non-homologous end joining or COM-1 attenuates the recruitment of MRE-11 through distinct mechanisms. The synaptonemal complex plays both positive and negative regulatory roles in these mutant contexts. These findings indicate that MRE-11 is regulated by modifying its accessibility to chromosomes.

Synaptonemal complex

C. elegans

Meiosis

Microirradiation

Complex damage/DNA repair

Details

Title: Subtitle: Recruitment of MRE-11 to complex DNA damage is modulated by meiosis-specific chromosome organization
Creators: Kailey Harrell - Department of Biology, University of Iowa, Iowa City, IA, 52241, USA
Madison Day - Department of Biology, University of Iowa, Iowa City, IA, 52241, USA
Sarit Smolikove - Department of Biology, University of Iowa, Iowa City, IA, 52241, USA. Electronic address: sarit-smolikove@uiowa.edu
Resource Type: Journal article
Publication Details: Mutation research, Vol.822, pp.111743-111743
DOI: 10.1016/j.mrfmmm.2021.111743
PMID: 33975127
PMCID: PMC8176624
NLM abbreviation: Mutat Res
ISSN: 0027-5107
eISSN: 1873-135X
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Date published: 04/20/2021
Academic Unit: Biology
Record Identifier: 9984217412202771

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