Journal article
Red-shifted emission from 1,2-dioxetane-based chemiluminescent reactions
Luminescence (Chichester, England), Vol.29(6), pp.553-558
09/2014
DOI: 10.1002/bio.2666
PMID: 24760607
Abstract
Commercial chemiluminescent reagents emit across a broad portion of the electromagnetic spectrum (400-500 nm). A challenge to the use of chemiluminescence to monitor biological processes is the presence of interfering substances in the biological optical window. In the present study, longer wavelength emitting fluorophores (the organic dyes Alexa 568 and Alexa 647), and a semiconductor nanoparticle (QDOT800) were used to red-shift the emission from commercially available 1,2-dioxetane-based chemiluminescent substrate reactions. By adding non-conjugated fluorescent emitters into chemiluminescent reaction mixtures, an emission peak occurred at the predicted wavelength of the fluorescent emitter. The excitation and emission from QDOT800 was preserved in the presence of a 100 µm-thick glass barrier separating it from the chemiluminescent reaction components. The maximum tissue phantom penetration by QDOT800 emission was 8.5 mm; in comparison, the native chemiluminescent emission at 500 nm was unable to penetrate the thinnest tissue phantom of 2.5 mm. The described method for red-shifted emissions from chemiluminescent reactions does not require direct interaction between the chemiluminescent reaction and the fluorescent emitters. This suggests that the mechanism of chemiluminescent excitation of fluorophores and QDOT800 is not exclusive to chemiluminescence resonance energy transfer or sensitized chemiluminescence, but rather by broad energization from the native chemiluminescent emission.
Details
- Title: Subtitle
- Red-shifted emission from 1,2-dioxetane-based chemiluminescent reactions
- Creators
- Jason Y Park - Department of Pathology, University of Texas Southwestern Medical Center and Children's Medical Center, Dallas, TX, USA; Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Dallas, TX, USAJoshua GunpatLi LiuBrooks EdwardsAlana ChristieXian-Jin XieLarry J KrickaRalph P Mason
- Resource Type
- Journal article
- Publication Details
- Luminescence (Chichester, England), Vol.29(6), pp.553-558
- Publisher
- England
- DOI
- 10.1002/bio.2666
- PMID
- 24760607
- ISSN
- 1522-7235
- eISSN
- 1522-7243
- Grant note
- We acknowledge the assistance of the Southwestern Small Animal Imaging Resource, which is supported in part by NCI Cancer Center Support Grant, 1P30 CA142543. The IVIS Spectrum was purchased under NIH 1S10RR024757. [Correction added on August 6, 2014, after first online publication: The author added an Acknowledgment section.]
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Preventive and Community Dentistry; Biostatistics; Dental Research
- Record Identifier
- 9983917768302771
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