Reduced Neuronal Self-Avoidance in Mouse Starburst Amacrine Cells With Only One Pcdhg Isoform

Cathy M McLeod; Seoyoung Son; Muhammad Nazmul Haque; Camille M Hanes; Andrew M Garrett

doi:10.1002/dneu.70005

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Reduced Neuronal Self-Avoidance in Mouse Starburst Amacrine Cells With Only One Pcdhg Isoform

Journal article

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Reduced Neuronal Self-Avoidance in Mouse Starburst Amacrine Cells With Only One Pcdhg Isoform

Cathy M McLeod, Seoyoung Son, Muhammad Nazmul Haque, Camille M Hanes and Andrew M Garrett

Developmental neurobiology (Hoboken, N.J.), Vol.86(1), e70005

01/2026

DOI: 10.1002/dneu.70005

PMCID: PMC12749552

PMID: 41466490

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Abstract

The clustered protocadherins (cPcdhs) are a family of ∼60 homophilic cell adhesion molecules expressed across three gene clusters (Pcdha, Pcdhb, and Pcdhg) with a variety of essential roles in the developing nervous system. Some of these roles rely on specific isoforms, whereas others are more consistent with a model of isoform redundancy or a requirement for diversity. The γ-Pcdhs (expressed from the Pcdhg gene cluster) are particularly important for neuronal self-avoidance in starburst amacrine cells in the mouse retina. Here, we used mouse mutants to test two of the C-type isoforms-γC4 and γC5-and found that neither was required for normal self-avoidance. Conversely, when we analyzed a mutant with only γC4 intact, we found significant failures in self-avoidance that could not be completely rescued by overexpression of this isoform from a transgene. We have recently found that this isoform is essential for normal neuronal survival during development, and our new findings here support the hypothesis that γC4 is specialized for the survival function at the expense of a significant role in self-avoidance.

Amacrine Cells - metabolism

Amacrine Cells - physiology

Animals

Avoidance Learning - physiology

Cadherin Related Proteins

Cadherins - genetics

Cadherins - metabolism

Mice

Mice, Inbred C57BL

Mice, Transgenic

Protein Isoforms - genetics

Protein Isoforms - metabolism

Retina - cytology

Retina - metabolism

Details

Title: Subtitle: Reduced Neuronal Self-Avoidance in Mouse Starburst Amacrine Cells With Only One Pcdhg Isoform
Creators: Cathy M McLeod - Wayne State University
Seoyoung Son - Wayne State University
Muhammad Nazmul Haque - Wayne State University
Camille M Hanes - University of Iowa
Andrew M Garrett - Wayne State University
Resource Type: Journal article
Publication Details: Developmental neurobiology (Hoboken, N.J.), Vol.86(1), e70005
DOI: 10.1002/dneu.70005
PMID: 41466490
PMCID: PMC12749552
NLM abbreviation: Dev Neurobiol
ISSN: 1932-846X
eISSN: 1932-846X
Publisher: Wiley
Grant note: Knights Templar Eye Foundation Research to Prevent Blindness NEI NIH HHS
Language: English
Date published: 01/2026
Academic Unit: Biology
Record Identifier: 9985112978102771

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