Reduced growth cone motility in cultured neurons from Drosophila memory mutants with a defective cAMP cascade

Y T Kim; C F Wu

doi:10.1523/jneurosci.16-18-05593.1996

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Reduced growth cone motility in cultured neurons from Drosophila memory mutants with a defective cAMP cascade

Journal article

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Reduced growth cone motility in cultured neurons from Drosophila memory mutants with a defective cAMP cascade

Y T Kim and C F Wu

The Journal of neuroscience, Vol.16(18), pp.5593-5602

09/15/1996

DOI: 10.1523/jneurosci.16-18-05593.1996

PMID: 8795615

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Abstract

Drosophila memory mutants dunce (dnc) and rutabaga (rut) are known to have altered intracellular cAMP levels, nerve terminal growth, and plasticity of synaptic transmission. Because the growth cone is responsible for neurite outgrowth and synaptogenesis, video microscopy was used to examine growth cone morphology and behavior of mutant neurons in larval CNS cultures. We found that growth cone exploratory movement was nearly arrested by both mutations, even though they change cAMP levels in opposite directions. The dnc phenotype could be mimicked by normal neurons when perfused with dibutyryl cAMP (db-cAMP) or forskolin. In contrast, rut growth cones became active when perfused with db-cAMP. Furthermore, motility was also restored by counterbalancing the effects of the two genes in double mutants, indicating that dynamic control of growth cone motility in developing Drosophila neurons requires optimal cAMP levels within an operational range. These findings represent the first demonstration of altered growth cone properties in learning and memory mutants and establish in a natural setting the role of cAMP in growth cone motility and neuronal plasticity.

Mutation

Central Nervous System - ultrastructure

Neurites - physiology

Colforsin - pharmacology

Cells, Cultured

Drosophila melanogaster - genetics

Central Nervous System - physiology

Animals

Central Nervous System - cytology

Larva

Neurites - ultrastructure

Neurons - physiology

Bucladesine - pharmacology

Cyclic AMP - metabolism

Neurites - drug effects

Memory - physiology

Details

Title: Subtitle: Reduced growth cone motility in cultured neurons from Drosophila memory mutants with a defective cAMP cascade
Creators: Y T Kim - Department of Biological Sciences, University of lowa, lowa City 52242, USA
C F Wu
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.16(18), pp.5593-5602
DOI: 10.1523/jneurosci.16-18-05593.1996
PMID: 8795615
NLM abbreviation: J Neurosci
ISSN: 0270-6474
eISSN: 1529-2401
Publisher: United States
Language: English
Date published: 09/15/1996
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984070751502771

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