Reduced-illuminance autofluorescence imaging in ABCA4-associated retinal degenerations

Artur V Cideciyan; Malgorzata Swider; Tomas S Aleman; Marisa I Roman; Alexander Sumaroka; Sharon B Schwartz; Edwin M Stone; Samuel G Jacobson

doi:10.1364/JOSAA.24.001457

Back

Reduced-illuminance autofluorescence imaging in ABCA4-associated retinal degenerations

Journal article

Open access

Peer reviewed

Reduced-illuminance autofluorescence imaging in ABCA4-associated retinal degenerations

Artur V Cideciyan, Malgorzata Swider, Tomas S Aleman, Marisa I Roman, Alexander Sumaroka, Sharon B Schwartz, Edwin M Stone and Samuel G Jacobson

Journal of the Optical Society of America. A, Optics, image science, and vision, Vol.24(5), pp.1457-1467

05/2007

DOI: 10.1364/JOSAA.24.001457

PMCID: PMC2579898

PMID: 17429493

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/2579898View

Open Access

Abstract

The health of the retinal pigment epithelium (RPE) can be estimated with autofluorescence (AF) imaging of lipofuscin, which accumulates as a byproduct of retinal exposure to light. Lipofuscin may be toxic to the RPE, and its toxicity may be enhanced by short-wavelength (SW) illumination. The high-intensity and SW excitation light used in conventional AF imaging could, at least in principle, increase the rate of lipofuscin accumulation and/or increase its toxicity. We considered two reduced-illuminance AF imaging (RAFI) methods as alternatives to conventional AF imaging. RAFI methods use either near-infrared (NIR) light or reduced-radiance SW illumination for excitation of fluorophores. We quantified the distribution of RAFI signals in relation to retinal structure and function in patients with the prototypical lipofuscin accumulation disease caused by mutations in ABCA4. There was evidence for two subclinical stages of macular ABCA4 disease involving hyperautofluorescence of both SW- and NIR-RAFI with and without associated loss of visual function. Use of RAFI methods and microperimetry in future clinical trials involving lipofuscinopathies should allow quantification of subclinical disease expression and progression without subjecting the diseased retina/RPE to undue light exposure.

Genetic Predisposition to Disease - genetics

Reproducibility of Results

Retinal Degeneration - genetics

Humans

Middle Aged

Male

Retinal Degeneration - metabolism

ATP-Binding Cassette Transporters - genetics

Microscopy, Fluorescence - methods

Sensitivity and Specificity

Adult

Female

Image Enhancement - methods

Retinoscopy - methods

Retinal Degeneration - pathology

Lipofuscin - metabolism

Lighting - methods

Details

Title: Subtitle: Reduced-illuminance autofluorescence imaging in ABCA4-associated retinal degenerations
Creators: Artur V Cideciyan - Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania, Philadelphia 19104, and Howard Hughes Medical Institute, University of Iowa Hospitals and Clinics, Iowa City 52242, USA. cideciya@mail.med.upenn.edu
Malgorzata Swider
Tomas S Aleman
Marisa I Roman
Alexander Sumaroka
Sharon B Schwartz
Edwin M Stone
Samuel G Jacobson
Resource Type: Journal article
Publication Details: Journal of the Optical Society of America. A, Optics, image science, and vision, Vol.24(5), pp.1457-1467
DOI: 10.1364/JOSAA.24.001457
PMID: 17429493
PMCID: PMC2579898
NLM abbreviation: J Opt Soc Am A Opt Image Sci Vis
ISSN: 1084-7529
eISSN: 1520-8532
Publisher: United States
Grant note: R01 EY013203-03 / NEI NIH HHS R01 EY013203-04 / NEI NIH HHS R01 EY013203 / NEI NIH HHS R01 EY013203-05 / NEI NIH HHS R01 EY013203-06 / NEI NIH HHS R01 EY013203-07 / NEI NIH HHS EY-013203 / NEI NIH HHS R01 EY013203-01 / NEI NIH HHS R01 EY013203-02 / NEI NIH HHS
Language: English
Date published: 05/2007
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983979912502771

Metrics

29 Record Views

132 Times Cited - Web of Science