Journal article
Reduced superhelicity of plasmid DNA produced by the rho-15 mutation in Escherichia coli
Molecular & general genetics, Vol.204(3), pp.424-429
09/1986
DOI: 10.1007/BF00331019
PMID: 3020379
Abstract
The plasmid pJSF6, a derivative of pBR327, could be maintained at 30° C in strains of Escherichia coli containing the strong rho mutation, rho-15. Plasmids extracted from rho-15 cells were always less negatively supercoiled than plasmids from rho+cells. Transduction experiments designed to separate the rho gene from possible extragenic suppressors showed that the rho allele consistently determined the degree of plasmid superhelicity. Comparison of the superhelicity of plasmids extracted from the rho-15 and from a gyrB mutant showed that at 30° C the negative supercoiling was reduced by the amounts ΔWrho=4.0±0.3 and ΔWgyr=6.0±0.3 turns; the effect of the rho-15 mutation on supercoiling was thus comparable to that of the gyrB mutation. A similar effect of the rho-15 mutation on the superhelicity of pBR329 was observed. The observation that the Rho protein has a role in determining DNA superhelicity (though not necessarily a direct role) provides a new point of view for studying the pleiotropic properties of rho mutants. © 1986 Springer-Verlag.
Details
- Title: Subtitle
- Reduced superhelicity of plasmid DNA produced by the rho-15 mutation in Escherichia coli
- Creators
- Jan S FasslerGail Ferstandig ArnoldIrwin Tessman
- Resource Type
- Journal article
- Publication Details
- Molecular & general genetics, Vol.204(3), pp.424-429
- DOI
- 10.1007/BF00331019
- PMID
- 3020379
- ISSN
- 0026-8925
- eISSN
- 1432-1874
- Language
- English
- Date published
- 09/1986
- Academic Unit
- Biology
- Record Identifier
- 9984217429402771
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