Journal article
Reducing the cytotoxicity of inhalable engineered nanoparticles via in situ passivation with biocompatible materials
Journal of hazardous materials, Vol.292, pp.118-125
07/15/2015
DOI: 10.1016/j.jhazmat.2015.03.022
PMID: 25797930
Abstract
[Display omitted]
•The cytotoxicity of model welding particles was modulated through in situ passivation.•Model welding particles were incorporated with chitosan nanoparticles for passivation.•In vitro assay revealed that the passivated particles had a lower cytotoxicity.•Passivation with chitosan adhesive or graphite paste could also reduce cytotoxicity.•This method would be suitable for efficient reduction of inhalable toxic components.
The cytotoxicity of model welding nanoparticles was modulated through in situ passivation with soluble biocompatible materials. A passivation process consisting of a spark discharge particle generator coupled to a collison atomizer as a co-flow or counter-flow configuration was used to incorporate the model nanoparticles with chitosan. The tested model welding nanoparticles are inhaled and that A549 cells are a human lung epithelial cell line. Measurements of in vitro cytotoxicity in A549 cells revealed that the passivated nanoparticles had a lower cytotoxicity (>65% in average cell viability, counter-flow) than the untreated model nanoparticles. Moreover, the co-flow incorporation between the nanoparticles and chitosan induced passivation of the nanoparticles, and the average cell viability increased by >80% compared to the model welding nanoparticles. As a more convenient way (additional chitosan generation and incorporation devices may not be required), other passivation strategies through a modification of the welding rod with chitosan adhesive and graphite paste did also enhance average cell viability (>58%). The approach outlined in this work is potentially generalizable as a new platform, using only biocompatible materials in situ, to treat nanoparticles before they are inhaled.
Details
- Title: Subtitle
- Reducing the cytotoxicity of inhalable engineered nanoparticles via in situ passivation with biocompatible materials
- Creators
- Jeong Hoon Byeon - School of Mechanical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of KoreaJae Hong Park - Department of Occupational and Environmental Health, University of Iowa, IA 52242, United StatesThomas M Peters - Department of Occupational and Environmental Health, University of Iowa, IA 52242, United StatesJeffrey T Roberts - Department of Chemistry, Purdue University, IN 47907, United States
- Resource Type
- Journal article
- Publication Details
- Journal of hazardous materials, Vol.292, pp.118-125
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jhazmat.2015.03.022
- PMID
- 25797930
- ISSN
- 0304-3894
- eISSN
- 1873-3336
- Language
- English
- Date published
- 07/15/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Occupational and Environmental Health
- Record Identifier
- 9983997999902771
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