Reduction of XNkx2-10 expression leads to anterior defects and malformation of the embryonic heart

Bryan G Allen; Kristina Allen-Brady; Daniel L Weeks

doi:10.1016/j.mod.2006.07.008

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Reduction of XNkx2-10 expression leads to anterior defects and malformation of the embryonic heart

Journal article

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Reduction of XNkx2-10 expression leads to anterior defects and malformation of the embryonic heart

Bryan G Allen, Kristina Allen-Brady and Daniel L Weeks

Mechanisms of development, Vol.123(10), pp.719-729

2006

DOI: 10.1016/j.mod.2006.07.008

PMCID: PMC2094041

PMID: 16949797

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Abstract

Normal vertebrate heart development depends upon the expression of homeodomain containing proteins related to the Drosophila gene, tinman. In Xenopus laevis, three such genes have been identified in regions that will eventually give rise to the heart, XNkx2-3, XNkx2-5 and XNkx2-10. Although the expression domains of all three overlap in early development, distinctive differences have been noted. By the time the heart tube forms, there is little XNkx2-10 mRNA detected by in situ analysis in the embryonic heart while both XNkx2-3 and XNkx2-5 are clearly present. In addition, unlike XNkx2-3 and XNkx2-5, injection of XNkx2-10 mRNA does not increase the size of the embryonic heart. We have reexamined the expression and potential role of XNkx2-10 in development via oligonucleotide-mediated reduction of XNkx2-10 protein expression. We find that a decrease in XNkx2-10 leads to a broad spectrum of developmental abnormalities including a reduction in heart size. We conclude that XNkx2-10, like XNkx2-3 and XNkx2-5, is necessary for normal Xenopus heart development.

Nkx

tinman

Congenital defects

Xenopus heart development

Details

Title: Subtitle: Reduction of XNkx2-10 expression leads to anterior defects and malformation of the embryonic heart
Creators: Bryan G Allen - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Kristina Allen-Brady - Department of Biomedical Informatics, University of Utah, Salt Lake City, UT 84108, USA
Daniel L Weeks - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Mechanisms of development, Vol.123(10), pp.719-729
DOI: 10.1016/j.mod.2006.07.008
PMID: 16949797
PMCID: PMC2094041
NLM abbreviation: Mech Dev
ISSN: 0925-4773
eISSN: 1872-6356
Publisher: Elsevier Ireland Ltd
Language: English
Date published: 2006
Academic Unit: Stead Family Department of Pediatrics; Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024532702771

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