Journal article
Refinement of cg05575921 demethylation response in nascent smoking
Clinical epigenetics, Vol.12(1), pp.1-92
06/24/2020
DOI: 10.1186/s13148-020-00882-w
PMCID: PMC7315557
PMID: 32580755
Abstract
The initiation of adolescent smoking is difficult to detect using carbon monoxide or cotinine assays. Previously, we and others have shown that the methylation of cg05575921 is an accurate predictor of adult smoking status. But the dose and time dependency of the demethylation response to smoking initiation in adolescents is not yet well understood. To this end, we conducted three consecutive annual in-person interviews and biological samplings of 448 high school students (wave 1 (W1)-wave 3 (W3)). At W1 (
n
= 448), 62 subjects reported using tobacco and 72 subjects reported using cannabis at least once in their life-time with 38 and 20 subjects having a positive cotinine and cannabinoid levels, respectively, at W1 intake. At W3 (
n
= 383), 67 subjects reported using tobacco and 60 subjects reported using cannabis at least once with 75 and 60 subjects having positive cotinine and cannabinoid levels, respectively, at W3. Subjects with undetectable cotinine levels at all three-time waves had stable levels of cg05575921 methylation throughout the study (88.7% at W1 and 88.8% at W3,
n
= 149), while subjects with positive cotinine levels at all 3 time points manifested a steady decrease in cg05575921 methylation (81.8% at W1 and 71.3% at the W3,
n
= 12). In those subjects with an affirmative smoking self-report at W3 (
n
= 17), the amount of demethylation at cg05575921 was correlated with time and intensity of smoking. We conclude that cg05575921 methylation is a sensitive, dose-dependent indicator of early stages of smoking, and may help to identify smokers in the early stages of smoking.
Details
- Title: Subtitle
- Refinement of cg05575921 demethylation response in nascent smoking
- Creators
- Kelsey Dawes - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAAllan Andersen - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAEmma Papworth - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USABrandon Hundley - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USANatasha Hutchens - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAHeba El Manawy - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAAshley Becker - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USALuke Sampson - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAWillem Philibert - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USAFrederick X Gibbons - Storrs, CT USAMeg Gerrard - Storrs, CT USARobert Philibert - Rm 2-426 MEB, 500 Newton Road, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Clinical epigenetics, Vol.12(1), pp.1-92
- DOI
- 10.1186/s13148-020-00882-w
- PMID
- 32580755
- PMCID
- PMC7315557
- NLM abbreviation
- Clin Epigenetics
- ISSN
- 1868-7075
- eISSN
- 1868-7083
- Publisher
- BioMed Central; London
- Grant note
- R01DA037648 / ;
- Language
- English
- Date published
- 06/24/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984066347402771
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