Journal article
Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model
Journal of controlled release, Vol.248, pp.53-59
02/28/2017
DOI: 10.1016/j.jconrel.2017.01.008
PMCID: PMC5305420
PMID: 28069556
Abstract
Bone fracture healing impairment related to systemic diseases such as diabetes can be addressed by growth factor augmentation. We previously reported that growth factors such as fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) work synergistically to encourage osteogenesis in vitro. In this report, we investigated if BMP-2 and FGF-2 together can synergistically promote bone repair in a leporine model of diabetes mellitus, a condition that is known to be detrimental to union. We utilized two kinds of plasmid DNA encoding either BMP-2 or FGF-2 formulated into polyethylenimine (PEI) complexes. The fabricated nanoplexes were assessed for their size, charge, in vitro cytotoxicity, and capacity to transfect human bone marrow stromal cells (BMSCs). Using diaphyseal long bone radial defects in a diabetic rabbit model it was demonstrated that co-delivery of PEI-(pBMP-2+pFGF-2) embedded in collagen scaffolds resulted in a significant improvement in bone regeneration compared to PEI-pBMP-2 embedded in collagen scaffolds alone. This study demonstrated that scaffolds loaded with PEI-(pBMP-2+pFGF-2) could be an effective way of promoting bone regeneration in patients with diabetes. [Display omitted]
Details
- Title: Subtitle
- Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model
- Creators
- Behnoush Khorsand - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA, United StatesNate Nicholson - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesAnh-Vu Do - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA, United StatesJohn E Femino - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesJames A Martin - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesEmily Petersen - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesBrian Guetschow - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesDouglas C Fredericks - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, United StatesAliasger K Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA, United States
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.248, pp.53-59
- DOI
- 10.1016/j.jconrel.2017.01.008
- PMID
- 28069556
- PMCID
- PMC5305420
- NLM abbreviation
- J Control Release
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100001460, name: American Orthopaedic Foot & Ankle Society; name: Lyle and Sharon Bighley Professorship
- Language
- English
- Date published
- 02/28/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985965702771
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