Regional Difference in Colonic Motility Response to Electrical Field Stimulation in Guinea Pig

Jung Myun Kwak; Reji Babygirija; Irena Gribovskaja-Rupp; Toku Takahashi; Shigeru Yamato; Kirk Ludwig

doi:10.5056/jnm.2013.19.2.192

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Regional Difference in Colonic Motility Response to Electrical Field Stimulation in Guinea Pig

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Regional Difference in Colonic Motility Response to Electrical Field Stimulation in Guinea Pig

Jung Myun Kwak, Reji Babygirija, Irena Gribovskaja-Rupp, Toku Takahashi, Shigeru Yamato and Kirk Ludwig

Journal of neurogastroenterology and motility, Vol.19(2), pp.192-203

04/2013

DOI: 10.5056/jnm.2013.19.2.192

PMCID: PMC3644655

PMID: 23667750

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Abstract

Background/Aims In isolated guinea-pig colon, we investigated regional differences in peristalsis evoked by intrinsic electrical nerve stimulation. Methods Four colonic segments from mid and distal colon of Hartley guinea pigs, were mounted horizontally in an organ bath. Measurement of pellet propulsion time, intraluminal pressure, electrical field stimulation (EFS; 0.5 ms, 60 V, 10 Hz), and response of pharmacological antagonists, were performed to isolated segments of colon to determine the mechanisms underlying peristaltic reflexes evoked by focal electrical nerve stimuli. Results In fecal pellet propulsion study, the velocity of pellet propulsion was significantly faster in the distal colon and decreased gradually to the proximal part of the mid colon. Intraluminal pressure recording studies showed that luminal infusion initiated normal peristaltic contractions (PCs) in 82% trials of the distal colon, compared to that of mid colon. In response to EFS, the incidence of PCs was significantly increased in the distal colon in contrast, the incidence of non-peristaltic contractions (NPCs) was significantly higher in the middle-mid colon, distal-mid colon and distal colon, compared to that of proximal-mid colon. Addition of L-NAME into the bath increased the frequency of NPCs. EFS failed to cause any PCs or NPCs contractions in the presence of hexamethonium, atropine or tetrodotoxin. Conclusions This study has revealed that electrical nerve stimulation of distal colon is the most likely region to elicit a peristaltic wave, compared with the mid or proximal colon. Our findings suggest that EFS-evoked PCs can be modulated by endogenous nitric oxide.

Original

Nitric oxide

Anti-peristalsis

Colonic transit

Details

Title: Subtitle: Regional Difference in Colonic Motility Response to Electrical Field Stimulation in Guinea Pig
Creators: Jung Myun Kwak - Department of Surgery, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI, USA
Reji Babygirija - Department of Surgery, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI, USA
Irena Gribovskaja-Rupp - Department of Surgery, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI, USA
Toku Takahashi - Department of Surgery, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI, USA
Shigeru Yamato - Division of Gastroenterology, National Center of Neurology and Psychiatry, Tokyo, Japan
Kirk Ludwig - Department of Surgery, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI, USA
Resource Type: Journal article
Publication Details: Journal of neurogastroenterology and motility, Vol.19(2), pp.192-203
DOI: 10.5056/jnm.2013.19.2.192
PMID: 23667750
PMCID: PMC3644655
NLM abbreviation: J Neurogastroenterol Motil
ISSN: 2093-0879
eISSN: 2093-0887
Publisher: Korean Society of Neurogastroenterology and Motility
Language: English
Date published: 04/2013
Academic Unit: Surgery
Record Identifier: 9984051794702771

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