Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga

Brett Berke; Chun-Fang Wu

doi:10.1523/JNEUROSCI.22-11-04437.2002

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Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga

Journal article

Open access

Peer reviewed

Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga

Brett Berke and Chun-Fang Wu

The Journal of neuroscience, Vol.22(11), pp.4437-4447

06/01/2002

DOI: 10.1523/JNEUROSCI.22-11-04437.2002

PMID: 12040051

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Abstract

The dunce (dnc) and rutabaga (rut) mutations of Drosophila affect a cAMP-dependent phosphodiesterase and a Ca(2+)/CaM-regulated adenylyl cyclase, respectively. These mutations cause deficiencies in several learning paradigms and alter synaptic transmission, growth cone motility, and action potential generation. The cellular phenotypes either are Ca(2+) dependent (neurotransmission and motility) or mediate a Ca(2+) rise (action potential generation). However, interrelations among these defects have not been addressed. We have established conditions for fura-2 imaging of Ca(2+) dynamics in the "giant" neuron culture system of Drosophila. Using high K(+) depolarization of isolated neurons, we observed a larger, faster, and more dynamic response from the growth cone than the cell body. This Ca(2+) increase depended on an influx through Ca(2+) channels and was suppressed by the Na(+) channel blocker TTX. Altered cAMP metabolism by the dnc and rut mutations reduced response amplitude in the growth cone while prolonging the response within the soma. The enhanced spatial resolution of these larger cells allowed us to analyze Ca(2+) regulation within distinct domains of mutant growth cones. Modulation by a previous conditioning stimulus was altered in terms of response amplitude and waveform complexity. Furthermore, rut disrupted the distinction in Ca(2+) responses observed between the periphery and central domain of growth cones with motile filopodia.

Potassium - metabolism

Electric Stimulation

Calcium Channels - metabolism

Calcium - metabolism

Neurons - ultrastructure

Sodium Channels - metabolism

Fura-2

Neurons - metabolism

Tetrodotoxin - pharmacology

Neurons - drug effects

Cyclic AMP - metabolism

Drosophila

Cells, Cultured

Reaction Time - physiology

Calcium Channel Blockers - pharmacology

Action Potentials - physiology

Cell Compartmentation - physiology

Learning - physiology

Patch-Clamp Techniques

Animals

Growth Cones - metabolism

Growth Cones - ultrastructure

Pseudopodia - metabolism

Mutation

Sodium Channel Blockers

Fluorescent Dyes

Details

Title: Subtitle: Regional calcium regulation within cultured Drosophila neurons: effects of altered cAMP metabolism by the learning mutations dunce and rutabaga
Creators: Brett Berke - Interdisciplinary Program in Neuroscience and Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
Chun-Fang Wu
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.22(11), pp.4437-4447
Publisher: United States
DOI: 10.1523/JNEUROSCI.22-11-04437.2002
PMID: 12040051
ISSN: 0270-6474
eISSN: 1529-2401
Language: English
Date published: 06/01/2002
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984070635002771

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