Journal article
Regular physical activity prevents chronic pain by altering resident muscle macrophage phenotype and increasing interleukin-10 in mice
Pain (Amsterdam), Vol.157(1), pp.70-79
01/2016
DOI: 10.1097/j.pain.0000000000000312
PMCID: PMC4685958
PMID: 26230740
Abstract
Regular physical activity in healthy individuals prevents development of chronic musculoskeletal pain; however, the mechanisms underlying this exercise-induced analgesia are not well understood. Interleukin-10 (IL-10), an antiinflammatory cytokine that can reduce nociceptor sensitization, increases during regular physical activity. Since macrophages play a major role in cytokine production and are present in muscle tissue, we propose that physical activity alters macrophage phenotype to increase IL-10 and prevent chronic pain. Physical activity was induced by allowing C57BL/6J mice free access to running wheels for 8 weeks and compared to sedentary mice with no running wheels. Using immunohistochemical staining of the gastrocnemius muscle to label regulatory (M2, secretes antiinflammatory cytokines) and classical (M1, secretes proinflammatory cytokines) macrophages, the percentage of M2-macrophages increased significantly in physically active mice (68.5% ± 4.6% of total) compared with sedentary mice (45.8% ± 7.1% of total). Repeated acid injections into the muscle enhanced mechanical sensitivity of the muscle and paw in sedentary animals, which does not occur in physically active mice; no sex differences occur in either sedentary or physically active mice. Blockade of IL-10 systemically or locally prevented the analgesia in physically active mice, ie, mice developed hyperalgesia. Conversely, sedentary mice pretreated systemically or locally with IL-10 had reduced hyperalgesia after repeated acid injections. Thus, these results suggest that regular physical activity increases the percentage of regulatory macrophages in muscle and that IL-10 is an essential mediator in the analgesia produced by regular physical activity.
Details
- Title: Subtitle
- Regular physical activity prevents chronic pain by altering resident muscle macrophage phenotype and increasing interleukin-10 in mice
- Creators
- Audrey Leung - aUniversity of Iowa Carver College of Medicine, Iowa City, IA, USAbNeuroscience Graduate Program, University of Iowa, Iowa City, IA, USADepartments of cInternal Medicine anddPhysical Therapy and Rehabilitation Science, University of Iowa Carver College of Medicine, Iowa City, IA, USANicholas S GregoryLee-Ann H AllenKathleen A Sluka
- Resource Type
- Journal article
- Publication Details
- Pain (Amsterdam), Vol.157(1), pp.70-79
- DOI
- 10.1097/j.pain.0000000000000312
- PMID
- 26230740
- PMCID
- PMC4685958
- NLM abbreviation
- Pain
- ISSN
- 0304-3959
- eISSN
- 1872-6623
- Publisher
- United States
- Grant note
- R01 AR061371 / NIAMS NIH HHS T32 NS045549 / NINDS NIH HHS T32GM007337 / NIGMS NIH HHS AR061371 / NIAMS NIH HHS T32 GM007337 / NIGMS NIH HHS T32NS045549 / NINDS NIH HHS
- Language
- English
- Date published
- 01/2016
- Academic Unit
- Infectious Diseases; Iowa Neuroscience Institute; Nursing; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040350502771
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