Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis

Benjamin Alleva; Nathan Balukoff; Amy Peiper; Sarit Smolikove

doi:10.1083/jcb.201606126

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Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis

Journal article

Open access

Peer reviewed

Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis

Benjamin Alleva, Nathan Balukoff, Amy Peiper and Sarit Smolikove

The Journal of cell biology, Vol.216(2), pp.393-408

02/2017

DOI: 10.1083/jcb.201606126

PMCID: PMC5294783

PMID: 28077446

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Abstract

In meiotic prophase I, homologous chromosome pairing is promoted through chromosome movement mediated by nuclear envelope proteins, microtubules, and dynein. After proper homologue pairing has been established, the synaptonemal complex (SC) assembles along the paired homologues, stabilizing their interaction and allowing for crossing over to occur. Previous studies have shown that perturbing chromosome movement leads to pairing defects and SC polycomplex formation. We show that FKB-6 plays a role in SC assembly and is required for timely pairing and proper double-strand break repair kinetics. FKB-6 localizes outside the nucleus, and in its absence, the microtubule network is altered. FKB-6 is required for proper movement of dynein, increasing resting time between movements. Attenuating chromosomal movement in fkb-6 mutants partially restores the defects in synapsis, in agreement with FKB-6 acting by decreasing chromosomal movement. Therefore, we suggest that FKB-6 plays a role in regulating dynein movement by preventing excess chromosome movement, which is essential for proper SC assembly and homologous chromosome pairing.

Signal Transduction

Phenotype

Mutation

Molecular Chaperones - metabolism

Caenorhabditis elegans Proteins - metabolism

Synaptonemal Complex - genetics

Immunophilins - genetics

RNA Interference

Time Factors

Immunophilins - metabolism

Cytoplasmic Dyneins - metabolism

Chromosome Positioning

Dyneins - metabolism

Caenorhabditis elegans - growth & development

Animals, Genetically Modified

Caenorhabditis elegans - genetics

Cytoplasmic Dyneins - genetics

Molecular Chaperones - genetics

Genotype

In Situ Hybridization, Fluorescence

Synaptonemal Complex - metabolism

Animals

Dyneins - genetics

Caenorhabditis elegans Proteins - genetics

Chromosome Pairing

Caenorhabditis elegans - enzymology

Microscopy, Fluorescence

Details

Title: Subtitle: Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis
Creators: Benjamin Alleva - Department of Biology, University of Iowa, Iowa City, IA 52242
Nathan Balukoff - Department of Biology, University of Iowa, Iowa City, IA 52242
Amy Peiper - Department of Biology, University of Iowa, Iowa City, IA 52242
Sarit Smolikove - Department of Biology, University of Iowa, Iowa City, IA 52242 sarit-smolikove@uiowa.edu
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.216(2), pp.393-408
Publisher: United States
DOI: 10.1083/jcb.201606126
PMID: 28077446
PMCID: PMC5294783
ISSN: 1540-8140
eISSN: 1540-8140
Grant note: P40 OD010440 / NIH HHS
Language: English
Date published: 02/2017
Academic Unit: Biology
Record Identifier: 9983992036402771

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