Journal article
Regulation of 14-3-3σ expression in human thyroid carcinoma is epigenetically regulated by aberrant cytosine methylation
Cancer letters, Vol.267(1), pp.165-174
2008
DOI: 10.1016/j.canlet.2008.03.017
PMCID: PMC2710378
PMID: 18440129
Abstract
Increased
14-3-3σ expression has been observed by immunohistochemistry in papillary and anaplastic tumors, but not follicular thyroid cancers.
14-3-3σ mRNA expression and methylation status was examined in tumor cell lines and primary thyroid tissues using real-time RT-PCR, bisulfite sequencing and methylation-specific PCR. Most of the 27 CpG’s in the gene’s CpG island were methylated in normal thyroid, TPC-1, NPA, FTC-238 and 2–7, which did not express 14-3-3σ. In contrast, they were unmethylated in KAK-1 and anaplastic lines KAT4 and DRO-90. 14-3-3σ expression was not increased in thyroid carcinomas, the majority of which had a methylated CpG island. In addition, 5-aza-dC treatment increased
14-3-3σ expression in the FTC-238 and NPA cell lines, which had low baseline expression. We conclude
14-3-3σ expression in thyroid carcinomas is regulated by CpG island hypermethylation.
Details
- Title: Subtitle
- Regulation of 14-3-3σ expression in human thyroid carcinoma is epigenetically regulated by aberrant cytosine methylation
- Creators
- Geeta Lal - Department of Surgery, Division of Surgical Oncology, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, 4641 JCP, Iowa city, IA 52242, USALakshmi Padmanabha - Department of Surgery, Division of Surgical Oncology, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, 4641 JCP, Iowa city, IA 52242, USAMatthew Provenzano - Department of Otolaryngology, University of Iowa, Iowa city, IA 52242, USAMatthew Fitzgerald - Department of Radiation Oncology and Free Radical and Radiation Oncology Program, University of Iowa, Iowa city, IA 52242, USAJamie Weydert - Department of Pathology, University of Iowa, Iowa city, IA 52242, USAFrederick E Domann - Department of Radiation Oncology and Free Radical and Radiation Oncology Program, University of Iowa, Iowa city, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cancer letters, Vol.267(1), pp.165-174
- DOI
- 10.1016/j.canlet.2008.03.017
- PMID
- 18440129
- PMCID
- PMC2710378
- NLM abbreviation
- Cancer Lett
- ISSN
- 0304-3835
- eISSN
- 1872-7980
- Publisher
- Elsevier BV
- Language
- English
- Date published
- 2008
- Academic Unit
- Stead Family Department of Pediatrics; Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984046815502771
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